Digestive Diseases and Sciences

, Volume 45, Issue 10, pp 1935–1943

Correlation Between Stellate Cell Activation and Serum Fibrosis Markers in Choline-Deficient l-Amino Acid-Defined Diet-Induced Rat Liver Fibrosis

  • Koji Hironaka
  • Isao Sakaida
  • Koichi Uchida
  • Kiwamu Okita
Article

DOI: 10.1023/A:1005556328028

Cite this article as:
Hironaka, K., Sakaida, I., Uchida, K. et al. Dig Dis Sci (2000) 45: 1935. doi:10.1023/A:1005556328028

Abstract

The aim of this study was to investigate the association of stellate cell activation with serum fibrosis markers in a rat model of hepatic fibrosis prepared using a choline-deficient l-amino acid (CDAA) defined diet. CDAA diet administration resulted in increased liver hydroxyproline contents in a time-dependent manner with activated stellate cells, expressing α-smooth muscle actin (α-SMA) as well as increased serum concentrations of amino-terminal procollagen type III peptide (PIIIP) and the 7S fragment of type IV collagen. Hydroxyproline content of the liver showed a closer correlation with the serum 7S (r = 0.75, P < 0.01) concentration than with the serum PIIIP (r = 0.51, P < 0.01) concentration. The percent area of α-SMA-positive cells showed stronger correlation with the serum PIIIP concentration (r = 0.85, P < 0.01) than with the 7S concentration (r = 0.50, P < 0.01). These results indicate that the serum PIIIP concentration reflects the activity of fibrogenesis, while the serum 7S concentration reflects the accumulation of collagen fibers in the liver.

hepatic fibrosis hepatic stellate cell serum fibrosis markers, α-smooth muscle actin 

Copyright information

© Plenum Publishing Corporation 2000

Authors and Affiliations

  • Koji Hironaka
    • 1
  • Isao Sakaida
    • 1
  • Koichi Uchida
    • 1
  • Kiwamu Okita
    • 1
  1. 1.First Department of Internal Medicine, School of MedicineYamaguchi University, UbeYamaguchi PrefectureJapan