Biotechnology Letters

, Volume 21, Issue 9, pp 791–795

Metabolic engineering of the terpenoid biosynthetic pathway of Escherichia coli for production of the carotenoids β-carotene and zeaxanthin

  • Manuela Albrecht
  • Norihiko Misawa
  • Gerhard Sandmann
Article

DOI: 10.1023/A:1005547827380

Cite this article as:
Albrecht, M., Misawa, N. & Sandmann, G. Biotechnology Letters (1999) 21: 791. doi:10.1023/A:1005547827380
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Abstract

Metabolic engineering of the early non-mevalonate terpenoid pathway of Escherichia coli was carried out to increase the supply of prenyl pyrophosphates as precursor for carotenoid production. Transformation with the genes dxs for over-expression of 1-deoxy-d-xylulose 5-phosphate synthase, dxr for 1-deoxy-d-xylulose 5-phosphate reductoisomerase and idi encoding an isopentenyl pyrophosphate stimulated carotenogenesis up to 3.5-fold. Co-transformation of idi with either dxs or dxr had an additive effect on ß-carotene and zeaxanthin production which reached 1.6 mg g−1 dry wt.

β-carotene carotenoid production engineered E. coli terpenoid biosynthesis zeaxanthin 

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Manuela Albrecht
    • 1
  • Norihiko Misawa
    • 2
  • Gerhard Sandmann
    • 1
  1. 1.Biosynthesis GroupBotanical Institute, Goethe UniversityFrankfurtGermany
  2. 2.Central Laboratories for Key TechnologyKirin BreweryYokohamaJapan

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