Journal of Chemical Ecology

, Volume 26, Issue 10, pp 2307–2319

Recognition of Complex Odors by Restrained and Free-Flying Honeybees, Apis mellifera

  • David Laloi
  • Omar Bailez
  • Margaret M. Blight
  • Bernard Roger
  • Minh-Ha Pham-Delègue
  • Lester J. Wadhams
Article

DOI: 10.1023/A:1005522826673

Cite this article as:
Laloi, D., Bailez, O., Blight, M.M. et al. J Chem Ecol (2000) 26: 2307. doi:10.1023/A:1005522826673

Abstract

Complex odor recognition in the honeybee was investigated using two behavioral assays: (1) the conditioning of the proboscis extension (CPE) with restrained individuals, and (2) the observation of foragers visiting an artificial feeder in a flight room. Nine compounds, previously identified as oilseed rape flower volatiles, were tested either individually or in mixtures. Different sets of experiments were done to determine: (1) the acquisition rate of the nine compounds in the CPE assay, and (2) the discrimination of the individual compounds after conditioning to a mixture, using the CPE assay and free-flying foragers. After conditioning to a complex mixture, honeybees established a hierarchy among the components, with some of them accounting for a major part of the behavioral activity of the mixture. Both behavioral assays led to the same classification of compounds, indicating good agreement between discriminating abilities of restrained individuals and of a population of foragers. The key compounds for recognition of these mixtures were those that were well learned when presented individually. However, the recognition of some compounds was affected by the other components of the mixture, with the activity of some compounds being either enhanced or reduced.

Honeybee Apis mellifera Hymenoptera Apidae conditioned proboscis extension free-flying foragers olfactory discrimination floral volatiles 

Copyright information

© Plenum Publishing Corporation 2000

Authors and Affiliations

  • David Laloi
    • 1
  • Omar Bailez
    • 1
    • 2
  • Margaret M. Blight
    • 3
  • Bernard Roger
    • 1
  • Minh-Ha Pham-Delègue
    • 1
  • Lester J. Wadhams
    • 3
  1. 1.Laboratoire de Neurobiologie Comparée des InvertébrésINRABures-sur-YvetteFrance
  2. 2.Facultad de Ciencias AgrariasUniversidad Nacional de Mar del PlataBalcarceArgentina
  3. 3.Department of Biological and Ecological ChemistryIACR-Rothamsted, HarpendenHertfordshireUK