Digestive Diseases and Sciences

, Volume 45, Issue 4, pp 723–729

Detection and Sequencing of Measles Virus from Peripheral Mononuclear Cells from Patients with Inflammatory Bowel Disease and Autism

  • Hisashi Kawashima
  • Takayuki Mori
  • Yasuyo Kashiwagi
  • Kouji Takekuma
  • Akinori Hoshika
  • Andrew Wakefield
Article

DOI: 10.1023/A:1005443726670

Cite this article as:
Kawashima, H., Mori, T., Kashiwagi, Y. et al. Dig Dis Sci (2000) 45: 723. doi:10.1023/A:1005443726670

Abstract

It has been reported that measles virus may be present in the intestine of patients with Crohn's disease. Additionally, a new syndrome has been reported in children with autism who exhibited developmental regression and gastrointestinal symptoms (autistic enterocolitis), in some cases soon after MMR vaccine. It is not known whether the virus, if confirmed to be present in these patients, derives from either wild strains or vaccine strains. In order to characterize the strains that may be present, we have carried out the detection of measles genomic RNA in peripheral mononuclear cells (PBMC) in eight patients with Crohn's disease, three patients with ulcerative colitis, and nine children with autistic enterocolitis. As controls, we examined healthy children and patients with SSPE, SLE, HIV-1 (a total of eight cases). RNA was purified from PBMC by Ficoll-paque, followed by reverse transcription using AMV; cDNAs were subjected to nested PCR for detection of specific regions of the hemagglutinin (H) and fusion (F) gene regions. Positive samples were sequenced directly, in nucleotides 8393–8676 (H region) or 5325–5465 (from noncoding F to coding F region). One of eight patients with Crohn disease, one of three patients with ulcerative colitis, and three of nine children with autism, were positive. Controls were all negative. The sequences obtained from the patients with Crohn's disease shared the characteristics with wild-strain virus. The sequences obtained from the patients with ulcerative colitis and children with autism were consistent with being vaccine strains. The results were concordant with the exposure history of the patients. Persistence of measles virus was confirmed in PBMC in some patients with chronic intestinal inflammation.

measlesinflammatory bowel diseasepervasive developmental disorder

Copyright information

© Plenum Publishing Corporation 2000

Authors and Affiliations

  • Hisashi Kawashima
    • 1
    • 2
    • 3
  • Takayuki Mori
    • 1
    • 2
    • 3
  • Yasuyo Kashiwagi
    • 1
    • 2
    • 3
  • Kouji Takekuma
    • 1
    • 2
    • 3
  • Akinori Hoshika
    • 1
    • 2
    • 3
  • Andrew Wakefield
    • 1
    • 2
    • 3
  1. 1.Department of PaediatricsTokyo Medical UniversityTokyoJapan
  2. 2.Department of VirologyKitasato InstituteTokyoJapan
  3. 3.Department of MedicineRoyal Free Hospital School of MedicineLondonUK