The Multifunctional Drosophila melanogaster V-ATPase Is Encoded by a Multigene Family
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- Dow, J.A.T. J Bioenerg Biomembr (1999) 31: 75. doi:10.1023/A:1005400731289
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In animals, V-ATPases are believed to play roles in the plasma membrane,as well as endomembrane. To understand these different functions, it isnecessary to adopt a genetic approach in a physiologically tractable modelorganism. For this purpose, Drosophila melanogaster is ideal,because of the powerful genetics associated with the organism and because ofthe unusually informative epithelial phenotype provided by the Malpighiantubule. Recently, the first animal “knockouts” of a V-ATPase weredescribed in Drosophila. The resulting phenotypes have generalutility for our understanding of V-ATPase function and suggest a screen fornovel subunits and associated proteins. Genome project resources haveaccelerated our knowledge of the V-ATPase gene family size and the newDrosophila genes vhaSFD, vha100-1, vha100-2, vha100-3, vha16-2,vha16-3, vha16-4, vhaPPA1, vhaPPA2, vhaM9.7.1, and vhaM9.7.2are described. The Drosophila V-ATPase model is thus well-suited toboth forward and reverse genetic analysis of this complex multifunctionalenzyme.