Biotechnology Letters

, Volume 20, Issue 8, pp 757–761

Trehalose synthesis from maltose by a thermostable trehalose synthase from Thermus caldophilus

  • Sukhoon Koh Koh
  • Hyun-Jae Shin
  • Joong Su Kim
  • Dae-Sil Lee
  • Se Young Lee
Article

DOI: 10.1023/A:1005342921339

Cite this article as:
Koh, S.K., Shin, HJ., Kim, J.S. et al. Biotechnology Letters (1998) 20: 757. doi:10.1023/A:1005342921339

Abstract

Purified trehalose synthase from Thermus caldophilus GK24 produced 18–86% trehalose from 10 mM–1 M maltose. The enzyme also catalyzed the conversion of α,α-trehalose into maltose but did not act on other disaccharides. The yield of trehalose from maltose by this enzyme increased 30% more at 40°C than at 80°C and was independent of the substrate concentration. The maximum yield of α,α-trehalose from 10 mM maltose reached 86% at 40°C. In addition, α,β-trehalose was also formed from maltose or α,α-trehalose at 3.5% yield at 80°C. © Rapid Science Ltd. 1998

Copyright information

© Chapman & Hall 1998

Authors and Affiliations

  • Sukhoon Koh Koh
    • 1
  • Hyun-Jae Shin
    • 1
  • Joong Su Kim
    • 1
  • Dae-Sil Lee
    • 1
  • Se Young Lee
    • 2
  1. 1.Molecular Glycobiology Research UnitKorea Research Institute of Bioscience and BiotechnologyYusong TaejonKorea
  2. 2.Graduate School of BiotechnologyKorea UniversityAnam-dong, Sungbook-gu SeoulKorea