The growth and phosphorus utilisation of plants in sterile media when supplied with inositol hexaphosphate, glucose 1-phosphate or inorganic phosphate Authors
Cite this article as: Hayes, J.E., Simpson, R.J. & Richardson, A.E. Plant and Soil (2000) 220: 165. doi:10.1023/A:1004782324030 Abstract
Seedlings of six temperate pasture species, three grasses and three legumes, were grown for 19–24 days in sterile agar or sand-vermiculite media, in the presence of inorganic phosphate (P
i), glucose 1-phosphate (G1P) or inositol hexaphosphate (IHP). Agar (pH 5.0) had a low IHP-sorbing capacity while IHP was almost completely sorbed in sand-vermiculite. P i and G1P were relatively available in both media. Growth of each species was measured in relation to phosphorus (P) supply and levels of P i supply at which shoot yields reached 90% of maximum yield (P crit) were determined. P crit values were generally higher for the legume species than for the grasses, and were six-fold higher for Trifolium subterraneum L. seedlings when grown in sand-vermiculite relative to agar. When supplied with G1P, seedlings of the six species grew as well as plants supplied with P i. By contrast, IHP was a poor source of P for plant growth, even when supplied in agar at levels up to 40-fold greater than P crit. Using the growth of T. subterraneum in the presence of IHP, it was calculated that roots released approximately 0.09 nkat phytase g -1 root dry wt per day, over 20 days of growth. By supplementing agar containing IHP with phytase from Aspergillus niger (E.C. 22.214.171.124; 0.012 nkat plant -1, or ∼1.3 nkat g -1 root dry wt), sufficient P became available to enable T. subterraneum seedlings to grow as well as P i-supplied plants. These results indicate that while pasture plants can quite effectively use P from some organic P sources (e.g. G1P), the acquisition of phytate-P is limited both by availability of substrate and the capacity of plant roots to hydrolyse available IHP. glucose 1-phosphate (G1P) inorganic phosphate (P i) inositol hexaphosphate (IHP phytate) P i growth response curves phosphorus nutrition phytase
This revised version was published online in June 2006 with corrections to the Cover Date.
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