The effects of nitrogen fertilisation and elevated CO2 on the lipid biosynthesis and carbon isotopic discrimination in birch seedlings (Betula pendula)
Purchase on Springer.com
$39.95 / €34.95 / £29.95*
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.
The effects of nitrogen (N) fertilisation and elevated [CO2] on lipid biosynthesis and carbon isotope discrimination in birch (Betula pendula Roth.) transplants were evaluated using seedlings grown with and without N fertiliser, and under two concentrations of atmospheric CO2 (ambient and ambient+250 μmol mol-1) in solar dome systems. N fertilisation decreased n-fatty acid chain length (18:0/16:0) and the ratios of α-linolenate (18:2)/linoleate (18:1), whereas elevated [CO2] showed little effect on n-fatty acid chain length, but decreased the unsaturation (18:2+18:1)/18:0. Both N fertilisation and elevated [CO2] increased the quantity of leaf wax n-alkanes, whilst reducing that of n-alkanols by 20–50%, but had no simple response in fatty acid concentrations. 13C enrichment by 1–2.5‰ under N fertilisation was observed, and can be attributed to both reduced leaf conductance and increased photosynthetic consumption of CO2. Individual n-alkyl lipids of different chain length show consistent pattern of δ13C values within each homologue, but are in general 5–8‰ more depleted in 13C than the bulk tissues. Niether nitrogen fertilisation and elevated CO2 influenced the relationship between carbon isotope discrimination of the bulk tissue and the individual lipids.
- Balesdent J, Wagner G H and Mariotti A 1988 Soil organic matter turnover in long-term field experiments as revealed by carbon-13 natural abundance. Soil Sci. Soc. Am. J. 52, 118–124. CrossRef
- Bender M M and Berge A J 1979 Influence of N and K fertilization and growth temperature on 13C/12C ratios of Timothy (Phleum pratense L.). Oecologia 44, 117–118.
- Brassell S C 1993 Applications of biomarkers for delineating marine paleoclimatic fluctuations during the pleistocene. In Organic Geochemistry. Eds M H Engel and S A Macko. Plenum Press, New York.
- Cerling T E, Harris J M, MacFadden B J, Leakey M G, Quade J, Eisenmann V and Ehleringer J R 1997 Global vegetation change through the Miocene/Pliocene boundary. Nature 389, 153–158. CrossRef
- Collister J W, Rieley G, Stern B, Eglinton G and Fry B 1994 Compound specific 13C analyses of leaf lipids from plants with differing carbon dioxide metabolism. Org. Geochem. 21, 619–628. CrossRef
- Cotrufo M F, Ineson P and Rowland A P 1994 Decomposition of tree leaf litters grown under elevated CO2: effect of litter quality. Plant Soil 163, 121–130.
- Cotrufo M F, Ineson P and Roberts J D 1995 Decomposition of birch leaf litters with varying C-to-N ratios. Soil Biol. Biochem. 27, 1219–1221. CrossRef
- Cranwell P A 1982 Lipids of aquatic sediments and sedimenting particulates. Prog. Lipid Res. 21, 271–308. CrossRef
- Delgado E, Mitchell R A C, Parry M A J, Driscoll S P, Mitchell V J and Lawalor D W 1994 Interacting effects of CO2 concentration, temperature and nitrogen supply on the photosynthesis and composition of winter wheat leaves. Plant Cell Environ. 17, 1205–1213. CrossRef
- Eglinton G and Hamilton R J 1963 The distribution of alkanes. In Chemical Plant Taxonomy. Ed. T Swain. pp. 187–217. Academic Press, London.
- Ehleringer J R 1991 13C/12C fractionation and its utility in terrestrial plant studies. In Carbon Isotope Techniques. Eds D C Coleman and B Fry. Academic press, London.
- Evans J R and von Caemmerer S 1996 Carbon dioxide diffusion inside leaves. Plant Physiol. 110, 339–346.
- Farquhar G D, Ehleringer J R and Hubick K T 1989 Carbon isotope discrimination and photosynthesis. Annu. Rev. Plant Physiol. Plant Mol. Biol. 40, 503–537. CrossRef
- Frey B, Scheidegger C, Günthardt-Goerg MS and Matyssek R 1996 The effects of ozone and nutrient supply on stomatal response in birch (Betula pendula) leaves as determined by digital imageanalysis and X-ray microanalysis. New Phytol. 132, 135–143. CrossRef
- Harwood J L and Russell N J 1983 Lipids in Plants and Microbes. George Allen & Unwin, London.
- Harwood J L 1994 Environmental factors which can alter lipid metabolism. Prog. Lipid Res. 33, 193–202. CrossRef
- Hayes J M 1993 Factors controlling 13C contents of sedimentary organic compounds: principles and evidence. Mar. Geol. 113, 111–125.
- Hayes J M, Freeman K H, Popp B N and Hoham C H 1990 Compound-specific isotopic analysis: a novel tool for reconstruction of ancient biogeochemical processes. Org. Geochem. 16, 1115–1128. CrossRef
- Huang Y, Lockheart M, Collister J W and Eglinton G 1995 Molecular and isotopic biogeochemistry of the Miocene Clarkia formation: hydrocarbons and alcohols. Org. Geochem. 23, 785–801. CrossRef
- Huang Y, Bol R, Harkness D D, Ineson P and Eglinton G 1996a Post-glacial variations in distributions, 13C and 14C contents of aliphatic hydrocarbons and bulk organic matter in three types of British acid upland soils. Org. Geochem. 24, 273–287. CrossRef
- Huang Y, Murray M, Metzger P and Eglinton G 1996b Novel unsaturated triterpenoid hydrocarbons from sediments of Sacred Lake, Mt. Kenya, Kenya. Tetrahedron 52, 6973–6982. CrossRef
- Huang Y, Eglinton G, Ineson P, Latter P M, Bol R and Douglas D D 1997 Absence of carbon isotope fractionation of individual n-alkanes in a 23 year field decomposition experiment with Calluna vulgaris. Org. Geochem. 26, 497–501. CrossRef
- Huang Y, Street-Perrott F A, Perrott F A, Metzger P and Eglinton G 1999 Glacial-interglacial environmental changes inferred from the molecular and compound-specific δ13C analyses of sediments from Sacred Lake, Mt Kenya. Geochim. Cosmochim. Acta 63, 1383–1404. CrossRef
- Jones D M, Carter J F, Eglinton G, Jumeau E J and Fenwick C S 1991 Determination of & #x03B4;13C values of sedimentary straight chain and cyclic alcohols by gas-chromatography isotope ratio massspectrometry. Biol. Mass Spectrometry 20, 641–646. CrossRef
- Kolattukudy P E, Croteau R and Buckner J S 1976 Biochemistry of plant waxes. In Chemistry and Biochemistry of Natural Waxes. Ed. P E Kolattukudy. pp. 289–347, Elsevier, Amsterdam.
- Lichtfouse É, Elbisser B, Balesdent J, Mariotti A and Bardoux G 1995 Isotope and molecular evidence for direct input of maize leaf wax n-alkanes into crop soils. Org. Geochem. 22, 349–351. CrossRef
- Monson K D and Hayes JM 1982 Biosynthetic control of the natural abundance of carbon 13 at specific positions within fatty acids in Saccharomyces cerevisiae. J. Biol. Chem. 257, 5568–5575.
- Morecroft M D, Woodward F I and Marrs R H 1992 Altitudinal trends in leaf nutrient contents, leaf size and & #x03B4;13C of Alchemilla alpina. Funct. Ecol. 6, 730–740. CrossRef
- O'Leary M H 1981 Carbon isotope fractionation in plants. Phytochemistry 20, 553–568. CrossRef
- O'Leary M H 1988 Carbon isotopes in photosynthesis. Bioscience 38, 328–336. CrossRef
- Raven J A and Farquhar G D 1990 The influence of N metabolism and organic acid synthesis on the natural abundance of isotopes of carbon in plants. New Phytol. 116, 505–529. CrossRef
- Rieley G, Collister J W, Stern B and Eglinton G 1993 Gas chromatography-isotope ratio mass spectrometry of leaf wax n alkanes from plants of differing carbon dioxide metabolisms. Rapid Commun. Mass Spectrom. 7, 488–491. CrossRef
- Schmidt G, Gebauer G, Widmann K and Ziegler H 1993 Influence of nitrogen supply and temperature on stable carbon isotope ratios in plants of different photosynthetic pathways (C3, C4, CAM). Isotopenpraxis 29, 9–13.
- Street-Perrott F A, Huang Y, Perrott A, Eglinton G, Baker P, Khelifa L, Harkness D D and Olago D 1997 The impact of lower atmospheric CO2 on tropical mountain ecosystems. Science 278, 1422–1426. CrossRef
- Townend, J.A. 1993 Effects of elevated carbon dioxide and drought on the growth and physiology of clonal Sitka spruce plants (Picea sitchensis (Bong.) Carr.). Tree Physiol. 13, 389–400.
- Watson R T, Rodhe H, Oescheger H and Siegenthalar U 1990 Greenhouse gases and aerosols. In Climatic Change: The IPCC Scientific Assessment. Eds J T Houghton, G J Jenkins and J J Ephraums. pp. 1–40. Cambridge University Press, Cambridge.
- Williams M, Shewry P R and Harwood J L 1994 The influence of the 'greenhouse effect' on wheat (Triticum aestivum L.) grain lipids. J. Exp. Bot. 45, 1379–1385.
- Williams M, Shewry P R and Harwood J L 1995 The effects of elevated temperature and atmospheric carbon-dioxide concentration on the quality of grain lipids in wheat (Triticum aestivum L.) grown at 2 levels of nitrogen application. Plant Cell Environ. 18, 999–1009. CrossRef
- Zhang F, Mackenzie A F and Smith D L 1993 Corn yield and shifts among corn quality constituents following application of different nitrogen fertilizer sources at several times during corn development. J. Plant Nutrition 16, 1317–1337. CrossRef
- Zhang F, Mackenzie A F and Smith D L 1994 Nitrogen fertilizer and protein, lipid, and non-structural carbohydrate concentrations during the course of maize kernel filling. J. Agron. Crop Sci. 172, 171–181.
- The effects of nitrogen fertilisation and elevated CO2 on the lipid biosynthesis and carbon isotopic discrimination in birch seedlings (Betula pendula)
Plant and Soil
Volume 216, Issue 1-2 , pp 35-45
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers
- Additional Links
- Betula pendula
- Nitrogen fertilisation
- CO2 concentration
- Carbon isotopic fractionation
- Industry Sectors
- Author Affiliations
- 1. Environmental and Analytical Chemistry Section, School of Chemistry, University of Bristol, Bristol, BS8 1TS, UK
- 2. Biogeochemistry Research Centre, c/o Department of Geology, University of Bristol, BS8 1RJ, UK
- 3. Soil Ecology Section, Merlewood Research Station, Windermere Road, Grange-Over-sands, Cumbria, LA11 6JU, UK
- 4. NERC Radiocarbon Laboratory, NEL Technology Park, East Kilbride, Glasgow, G75 0QU, UK