Plant and Soil

, Volume 221, Issue 1, pp 67-79

Modulation of amino acid metabolism in transformed tobacco plants deficient in Fd-GOGAT

  • S. Ferrario-Méry
  • , A. Suzuki
  • , C. Kunz
  • , M.H. Valadier
  • , Y. Roux
  • , B. Hirel
  • , C.H. Foyer

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Tobacco (Nicotiana tabacum) plants expressing a

partial ferredoxin-dependent glutamine-2-oxoglutarate aminotransferase (Fd-GOGAT) cDNA in the antisense orientation under the control of the 35S promoter, were used to study the metabolism of amino acids, 2-oxoglutarate and ammonium following the transition from CO2 enrichment (where photorespiration is inhibited) to air (where photorespiration is a major process of ammonium production in leaves). The leaves of the lowest Fd-GOGAT expressors accumulated more foliar glutamine (Gln) and α-ketoglutarate (α-KG) than the untransformed controls in both growth conditions. Photorespiration-dependent increases in foliar ammonium, glutamine, α-KG and total amino acids were proportional to the decreases in foliar Fd-GOGAT activity. No change in endoprotease activity was observed following transfer to air in the Fd-GOGAT transformants or the untransformed controls which has similar activities over a broad range of pH values. We conclude that several pathways of amino acid biosynthesis are modified when NH3 + and Gln accumulate in leaves.

α-ketoglutarate amino acid ammonium assimilation glutamate synthase Nicotiana tabacum transgenic plant