Nodulation restrictive genotypes of Glycine and Amphicarpaea: a comparative analysis
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Several genes that restrict nodulation with specific Bradyrhizobiumstrains are known in Glycine max (soybean), and a similar system of nodulation restriction has recently been discovered in the related North American legume Amphicarpaea bracteata. We analyzed how nodulation-restrictive genotypes of each plant interacted with Bradyrhizobium strains sampled from the other host species. Ten bacterial isolates from A. bracteata that nodulated differentially with genotypes of their homologous host legume showed uniform responses to two soybean isogenic lines that differed at the Rj4 locus controlling nodulation restriction: all isolates formed nodules of normal size and morphology on both isolines. However, little or no nitrogen fixation occurred in any of these symbioses. A. bracteata genotypes that displayed broad vs. restricted symbiotic phenotypes toward naturally-associated bradyrhizobia were also tested with two bacterial isolates from soybean (USDA 76 and USDA 123). Both isolates formed nodules and fixed nitrogen in association with both A. bracteata genotypes. However, symbiotic effectiveness (as measured by acetylene reduction assays) was normal only for the combination of USDA 76 with the restrictive A. bracteata genotype. Overall, these results indicate that plant genes that restrict nodulation by certain naturally-associated bradyrhizobia do not confer comparable specificity when plants interact with bacteria from another related legume species.
- Bottomley P J, Cheng H H and Strain S R 1994 Genetic structure and symbiotic characteristics of a Bradyrhizobiumpopulation recovered from a pasture soil. Appl. Environ. Microbiol. 60, 1754-1761.
- Burrill T J and Hansen R 1917 Is symbiosis possible between legume bacteria and non-legume plants? Univ. Illinois Agric. Exp. Stn. Bull. 202, 115-181.
- Conklin M E 1936 Studies of the root-nodule organisms of certain wild legumes. Soil Sci. 41, 167-185.
- Devine T E 1987 A comparison of rhizobial strain compatibilities of Glycine maxand its progenitor species Glycine soja. Crop Sci. 27, 635-639.
- Devine T E and Breithaupt B H 1980 Significance of incompatibility reactions of Rhizobium japonicumstrains with soybean host genotypes. Crop Sci. 20, 269-271.
- Devine T E, Kuykendall L D and Breithaupt B H 1983 Nodule-like structures induced on peanut by chlorosis producing strains of Rhizobium classifed as R. japonicum. Crop Sci. 23, 394-397.
- Devine T E, Kuykendall L D and O'Neill J J 1990 The Rj4allele in soybean represses nodulation by chlorosis-inducing bradyrhizobia classified as DNA homology group II by antibiotic resistance profiles. Theor. Appl. Genet. 80, 33-37.
- Devine T E and O'Neill J J 1986 Registration of BARC-2 (Rj4) and BARC-3 (rj4) soybean germplasm. Crop Sci. 27, 1263-1264.
- Devine T E and O'Neill J J 1989 Genetic allelism of nodulation response genes Rj1, Rj2, and Rj4in soybean. Crop Sci. 29, 1347- 135a.
- Doyle J J and Doyle J L 1993 Chloroplast DNA phylogeny of the papilionoid legume tribe Phaseoleae. Syst. Bot. 18, 309-327.
- Fuhrmann J 1990 Symbiotic effectiveness of indigenous soybean Bradyrhizobia as related to serological, morphological, rhizobitoxine and hydrogenase phenotypes. Appl. Environ. Microbiol. 56, 224-229.
- Holl F B 1975 Host plant control of the inheritance of dinitrogen fixation in the Pisum-Rhizobiumsymbiosis. Euphytica 24, 767- 770.
- Kneen B E and Larue T A 1984 Peas (Pisum sativumL.) with strain specificity for Rhizobium leguminosarum. Heredity 52, 383-389.
- Kuykendall L D, Saxena B, Devine T E and Udell S E 1992 Genetic diversity in Bradyrhizobium japonicumJordan 1982 and a proposal for Bradyrhizobium elkaniisp. nov. Can. J. Microbiol. 38, 501-505.
- Lackey J A 1977 A revised classification of the tribe Phaseoleae (Leguminosae: Paplionoideae), and its relation to canavanine distribution. Bot. J. Linn. Soc. 74, 163-178.
- Lie T A, Goktan D, Engin M, Pijnenborg J and Anlarsal E 1987 Coevolution of the legume-Rhizobiumassociation. Plant Soil 100, 171-181.
- Mitchell H L 1972 Microdetermination of nitrogen in plant tissues. J. Assoc. Anal. Chem. 55, 1-3.
- Nutman P S 1954 Symbiotic effectiveness in nodulated red clover. II. A major gene for ineffectiveness in the host. Heredity 8, 47-60.
- Parker MA and Wilkinson H H 1997 A locus controlling nodulation specificity in Amphicarpaea bracteata. J. Hered. (In press).
- Selander R K, Caugant D A, Ochman H, Musser J M, Gilmour M N and Whittam T S 1986 Methods for multilocus enzyme electrophoresis for bacterial population genetics and systematics. Appl. Environ. Microbiol. 51, 873-884.
- Sneath P H A and Sokal R R 1973 Numerical taxonomy. W H Freeman, San Francisco, CA. 573 p.
- Spoerke J M, Wilkinson H H and Parker M A 1996 Nonrandom genotypic associations in a legume-Bradyrhizobiummutualism. Evolution 50, 146-154.
- Turner B L and Fearing O S 1964 A taxonomic study of the genus Amphicarpaea (Leguminosae). Southwest. Nat. 9, 207-218.
- Vincent J M 1970 A manual for the practical study of the root-nodule bacteria. Blackwell, Oxford. 164 p.
- Wilkinson H H, Spoerke J M and Parker M A 1996 Divergence in symbiotic compatibility in a legume-Bradyrhizobiummutualism. Evolution 50, 1470-1477.
- Williams L F and Lynch D L 1954 Inheritance of a non-nodulating character in the soybean. Agron. J. 46, 28-29.
- Wilson J K 1939 Leguminous plants and their associated organisms. Cornell Univ. Agric. Exp. Stn. Mem. 221, 1-48.
- Zhu T, Shi L, Doyle J J and Keim P 1995 A single nuclear locus phylogeny of soybean based on DNA sequence. Theor. Appl. Genet. 90, 991-999.
- Nodulation restrictive genotypes of Glycine and Amphicarpaea: a comparative analysis
Plant and Soil
Volume 189, Issue 2 , pp 181-188
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- Author Affiliations
- 1. Department of Biological Sciences, State University of New York, Binghamton, NY, 13902, USA
- 2. Plant Molecular Biology Laboratory, Plant Sciences Institute, USDA-ARS, Beltsville Agricultural Research Center-West, Beltsville, MD, 20705, USA