, Volume 438, Issue 1, pp 99–105

Taxonomic re-evaluation of Aphanizomenon flos-aquae NH-5 based on morphology and 16S rRNA gene sequences

  • Renhui Li
  • Wayne W. Carmichael
  • Yongding Liu
  • Makoto M. Watanabe

DOI: 10.1023/A:1004166029866

Cite this article as:
Li, R., Carmichael, W.W., Liu, Y. et al. Hydrobiologia (2000) 438: 99. doi:10.1023/A:1004166029866


The taxonomy of Aphanizomenon flos-aquae strain NH-5, a producer of cyanotoxins, was re-evaluated by comparison with six other Aphanizomenon strains using morphological characteristics and 16S rRNA gene sequences. Strain NH-5 was concluded to be improperly identified as Aph. flos-aquae based upon (1) lack of bundle formation in the trichomes, (2) location of akinetes next to heterocytes, (3) lower similarities (less than 97.5%) in the 16S rRNA gene sequences relative to Aph. flos-aquae strains, and (4) comparison within a phylogenetic tree constructed from 16S rRNA gene sequences. The Aphanizomenon strains investigated in this study are classified to four morphological groups as described by the classical taxonomy of Komárek & Kovácik (1989). This classification was supported from the phylogenetic results of 16S rRNA gene sequences. This study also discusses the generic boundaries between Aphanizomenon and Anabaena.

AphanizomenonCyanobacteria16S rRNAmorphologysequencestaxonomy

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Renhui Li
    • 1
  • Wayne W. Carmichael
    • 2
  • Yongding Liu
    • 3
  • Makoto M. Watanabe
    • 4
  1. 1.Department of Biological SciencesWright State UniversityDaytonU.S.A.
  2. 2.Department of Biological SciencesWright State UniversityDaytonU.S.A.
  3. 3.Institute of Hydrobiologythe Chinese Academy of SciencesWuhanP. R. China
  4. 4.National Institute for Environmental StudiesIbarakiJapan