Article

Hydrobiologia

, Volume 430, Issue 1, pp 113-120

First online:

Immunostimulatory effect of leaf extract of Ocimum sanctum Linn. in Oreochromis mossambicus (Peters)

  • S. M. LogambalAffiliated withCentre for Fish Immunology, Postgraduate and Research Department of Zoology, The American College
  • , S. VenkatalakshmiAffiliated withCentre for Fish Immunology, Postgraduate and Research Department of Zoology, The American College
  • , R. Dinakaran MichaelAffiliated withCentre for Fish Immunology, Postgraduate and Research Department of Zoology, The American College

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Abstract

Effect of leaf extract of Ocimum sanctum on (i) the specific and non-specific immune responses and (ii) disease resistance against Aeromonas hydrophila was investigated in Oreochromis mossambicus. Sheep red blood cells (SRBC) and Heat aggregated bovine serum albumin (HA-BSA) were used as antigens for specific and non-specific immune response studies, respectively. Antigens were administered through intraperitoneal route. Anti-SRBC antibody titres were determined by direct haemagglutination and anti-bacterial antibodies were determined by bacterial agglutination. Nitroblue tetrazolium (NBT) assay was used to determine neutrophil activity. Disease resistance was measured in terms of relative percent survival (RPS). The immunostimulatory effect of the leaf extract of O. sanctum, when administered through intraperitoneal and oral routes was obvious. Leaf extract of O. sanctum, when administered intraperitoneally, stimulated both antibody response and neutrophil activity. Dietary intake of O. sanctum also enhanced the antibody response and disease resistance against A. hydrophila. Possibility of using O. sanctum as immunostimulant in the maintenance of finfish health in intensive freshwater aquaculture is suggested.

immunostimulation plant extracts fish antibody response neutrophil activity disease resistance