The Histochemical Journal

, Volume 31, Issue 12, pp 761–770

Stromelysin-1 (MMP-3) in Forming Enamel and Predentine in Rat Incisor – Coordinated Distribution with Proteoglycans Suggests a Functional Role

Authors

  • Rachel Hall
    • Basic Dental Science, Dental SchoolUniversity of Wales College of Medicine
  • Dominique Septier
    • Laboratoire de Biologie et Physiopathologie Cranio-faciales – EA 2496, Groupe Matrices Extracellulaires et Biominéralisations, Faculté de Chirurgie DentaireUniversité
  • Graham Embery
    • Basic Dental Science, Dental SchoolUniversity of Wales College of Medicine
  • Michel Goldberg
    • Laboratoire de Biologie et Physiopathologie Cranio-faciales – EA 2496, Groupe Matrices Extracellulaires et Biominéralisations, Faculté de Chirurgie DentaireUniversité
Article

DOI: 10.1023/A:1003945902473

Cite this article as:
Hall, R., Septier, D., Embery, G. et al. Histochem J (1999) 31: 761. doi:10.1023/A:1003945902473

Abstract

Stromelysin-1 (matrix metalloproteinase-3) or proteoglycanase was visualized by light and electron microscopy immunolabelling in the forming zone of rat incisors. In predentine, labelling was more dense at the transition zone between the inner proximal third and the two outer thirds. Odontoblast processes were also positively stained, mostly in predentine and to a lesser degree in dentine. The dentine–enamel junction was intensely labelled, whereas dentine and forming enamel were only faintly stained. Gold–antibodies complexes were seen inside secretory ameloblasts and odontoblasts in cytosolic locations. The distribution of stromelysin-1 was compared with the distribution of 2-B-6 epitope, an antibody recognizing chondroitin-4-sulphate/dermatan sulphate and which showed a decreasing gradient from the proximal zone to the distal part of predentine. In contrast, both 5-D-4, an anti-keratan sulphate antibody and an anti-lumican antibody displayed a reversed distribution, with an increase seen from the proximal and central thirds to the distal part of predentine. This coordinated distribution suggests that stromelysin-1 may have a functional role, being implicated in predentine in the degradation of chondroitin-4-sulphate/dermatan sulphate-containing proteoglycans, and consequently allowing keratan sulphate proteoglycan concentration to increase near the border where mineralization is initiated.

Copyright information

© Kluwer Academic Publishers 1999