Hydrobiologia

, Volume 358, Issue 1, pp 237–244

Culture of Tetraselmis tetrathele and its utilization in the hatchery production of different penaeid shrimps in Asia

  • Jesse D. Ronquillo
  • Jonathan R. Matias
  • Toshio Saisho
  • Shigehisa Yamasaki
Article

DOI: 10.1023/A:1003128701968

Cite this article as:
Ronquillo, J.D., Matias, J.R., Saisho, T. et al. Hydrobiologia (1997) 358: 237. doi:10.1023/A:1003128701968

Abstract

The prasinophyte Tetraselmis tetrathele is an important live feedorganism for shrimp/prawn hatchery operations because of its highnutritional value and ease of culture. It can replace Brachionus plicatilisand Artemia nauplii as live diet during the protozoeal and mysis feedingstage. This study tried to determine the different optimum cultureconditions for T. tetrathele, and to evaluate its application in thehatchery production of different penaeid species. Tetraselmis tetrathelewas cultured at combinations of six levels of salinities (10–60 gkg−1), nine levels of pH (3–10.5), and two temperatures(25°C and 30°C) in a three factorialcombination experiment. Effects of varying concentrations of differentorganic and inorganic media including the use of vitamins and trace metalswere also assessed. The dietary value of T. tetrathele was evaluated byfeeding it to different shrimp larvae from protozoea-1 (PZ-1) untilpostlarva-1 (PL-1). There was no significant difference (P > 0.05) inthe growth of T. tetrathele at 25°C and30°C in acidic media; but, there were significantdifferences (P < 0.05) in the range favoring fast growth at25°C. For the inorganic media test, the optimumconcentration for the maximum growth rate of T. tetrathele was at 1 gkg−1 of Yashima medium without urea (k = 0.57). Thegrowth rate for the other media was highly variable with some approximatingthe optimum. For inorganic nutrients, the optimum concentration for maximumgrowth was at 4 g kg−1 of unsterilized ’bio-conversion‘ mediumwith 30 mg l−1 of Clewat-32 (k = 0.72). The othergrowth rates also indicated significant positive rates but were far from theoptimum. The combination of T. tetrathele and Chaetoceros gracilis or C.calcitrans could be used as the only diet from protozoea-1 (PZ-1) untilpostlarva-1 (PL-1) for Metapenaeus ensis, Metapenaeopsis barbata,Trachypenaeus curvirostris, Penaeus indicus, P. merguiensis, P.latisulcatus, and P. japonicus, with high survival rates. However, thelarvae of P. semisulcatus, P. monodon and P.chinensis can benefit from apure phytoplanton diet until the second mysis stage (M-2) only. Beyond thisstage, the larvae need bigger zooplankton as live feed.

Tetraselmis tetrathelepenaeid shrimpsprawn hatcherylive feedslarval dietlarval rearingphytoplanktonculture

Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Jesse D. Ronquillo
    • 1
  • Jonathan R. Matias
    • 2
  • Toshio Saisho
    • 3
  • Shigehisa Yamasaki
    • 3
  1. 1.College of FisheriesUniversity of the Philippines in the VisayasMiagao, IloiloPhilippines
  2. 2.Nova Pacific Research InstituteUniversity of the Philippines in the VisayasIloiloPhilippines
  3. 3.Faculty of FisheriesKagoshima UniversityKagoshima CityJapan