Abstract
In vitro culture of long-day plant Chenopodium murale L was established. The effects of photoperiod, glucose and gibberellic acid (GA3) on flowering and growth in vitro were investigated. Oscillatory changes of photoperiodic sensitivity were noticeable with regard to plant age. The plants induced at the phase of the 1st and the 3rd pair of leaves flowered to higher degree than those induced at the phase of 2nd pair. Plants induced at the phase of the 1st pair of leaves flowered to 17 % on 5 % glucose-containing medium and the addition of 5 mg dm-3 GA3 resulted in maximum flowering (43 %). Neither glucose nor GA3 were able to compensate for photoperiodic requirements for flowering. Hypocotyl growth was decreased and the 1st internode elongation and development of leaves were increased due to inductive photoperiodic conditions, as compared to non-inductive ones.
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Mitrović, A., Živanović, B. & Ćulafić, L. The Effects of Photoperiod, Glucose and Gibberellic Acid on Growth In Vitro and Flowering of Chenopodium Murale . Biologia Plantarum 43, 173–177 (2000). https://doi.org/10.1023/A:1002727521624
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DOI: https://doi.org/10.1023/A:1002727521624