Antonie van Leeuwenhoek

, Volume 77, Issue 4, pp 329–335

Changes of cell size distribution during the batch culture of Arthrobacter strain PI/1-95

  • Christian E. Erlebach
  • Paul Illmer
  • Franz Schinner
Article

DOI: 10.1023/A:1002632128921

Cite this article as:
Erlebach, C.E., Illmer, P. & Schinner, F. Antonie Van Leeuwenhoek (2000) 77: 329. doi:10.1023/A:1002632128921

Abstract

The size distributions of an arthrobacter, approximately 1 μm in diameter, were analysed using a Coulter® Multisizer II instrument thereby making it possible to distinguish between the different stages in the morphological cycle. The results indicated that at the beginning of exponential phase a shift occurred from large to smaller cells, the cell size distributions in both categories were asymmetric, skewed towards higher values than the means. During the course of the batch culture the cells in the larger class decreased in size and the two classes coalesced. The size distribution of the smaller class exhibited an additional shift towards smaller cells. After which neither the number of classes nor the size distribution changed. It was concluded that the Coulter® Multisizer II instrument provides a feasible method to extract information not only about mean cell size but also about cell size distributions.

Arthrobacter strain PI/1-95cell sizeCoultersize distribution

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Christian E. Erlebach
    • 1
  • Paul Illmer
    • 2
  • Franz Schinner
    • 1
  1. 1.Department of Microbiology, Faculty of Natural SciencesUniversity of InnsbruckInnsbruckAustria
  2. 2.Department of Microbiology, Faculty of Natural SciencesUniversity of InnsbruckInnsbruckAustria