Antonie van Leeuwenhoek

, Volume 76, Issue 1, pp 367–376

Anchoring of proteins to lactic acid bacteria


DOI: 10.1023/A:1002095802571

Cite this article as:
Leenhouts, K., Buist, G. & Kok, J. Antonie Van Leeuwenhoek (1999) 76: 367. doi:10.1023/A:1002095802571


The anchoring of proteins to the cell surface of lactic acid bacteria (LAB) using genetic techniques is an exciting and emerging research area that holds great promise for a wide variety of biotechnological applications. This paper reviews five different types of anchoring domains that have been explored for their efficiency in attaching hybrid proteins to the cell membrane or cell wall of LAB. The most exploited anchoring regions are those with the LPXTG box that bind the proteins in a covalent way to the cell wall. In recent years, two new modes of cell wall protein anchoring have been studied and these may provide new approaches in surface display. The important progress that is being made with cell surface display of chimaeric proteins in the areas of vaccine development and enzyme- or whole-cell immobilisation is highlighted.

Lactococcus lactisLactobacillus subsp.Staphylococcus carnosusStaphylococcus xylosusStreptococcus gordoniisurface display

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  1. 1.Department of Genetics, Groningen Biomolecular Sciences and Biotechnology InstituteUniversity of GroningenHarenThe Netherlands