Biologia Plantarum

, Volume 41, Issue 3, pp 331–337

Direct Organogenesis in Hypocotyl Cultures of Tamarindus Indica

Authors

  • P.K. Jaiwal Sonia
    • Department of BioSciencesM.D. University
  • A. Gulati
    • Department of BioSciencesM.D. University
  • S. Dahiya
    • Department of BioSciencesM.D. University
Article

DOI: 10.1023/A:1001873604870

Cite this article as:
Sonia, P.J., Gulati, A. & Dahiya, S. Biologia Plantarum (1998) 41: 331. doi:10.1023/A:1001873604870

Abstract

Direct differentiation of shoot buds from hypocotyl segments of 12-d-old seedlings of Tamarindus indica was obtained on Murashige and Skoog (MS) medium with or without growth regulators. The highest regeneration (66 %) and the maximum number of shoots (3 - 4) per explant were obtained from the explants on MS medium containing 6-benzylaminopurine (5 × 10-6 M). A maximum roots per shoot were produced on medium containing 3-indole butyric acid (5 × 10-6 M). The resulting plantlets were hardened and transferred to soil in pots where 75 % of them survived and resumed growth. Histological examination of explants suggests that the shoots were of de novo origin which would make this system suitable for transformation experiments.

plantletsleguminous treetamarind

Copyright information

© Kluwer Academic Publishers 1998