Biologia Plantarum

, Volume 41, Issue 3, pp 331–337

Direct Organogenesis in Hypocotyl Cultures of Tamarindus Indica


  • P.K. Jaiwal Sonia
    • Department of BioSciencesM.D. University
  • A. Gulati
    • Department of BioSciencesM.D. University
  • S. Dahiya
    • Department of BioSciencesM.D. University

DOI: 10.1023/A:1001873604870

Cite this article as:
Sonia, P.J., Gulati, A. & Dahiya, S. Biologia Plantarum (1998) 41: 331. doi:10.1023/A:1001873604870


Direct differentiation of shoot buds from hypocotyl segments of 12-d-old seedlings of Tamarindus indica was obtained on Murashige and Skoog (MS) medium with or without growth regulators. The highest regeneration (66 %) and the maximum number of shoots (3 - 4) per explant were obtained from the explants on MS medium containing 6-benzylaminopurine (5 × 10-6 M). A maximum roots per shoot were produced on medium containing 3-indole butyric acid (5 × 10-6 M). The resulting plantlets were hardened and transferred to soil in pots where 75 % of them survived and resumed growth. Histological examination of explants suggests that the shoots were of de novo origin which would make this system suitable for transformation experiments.

plantletsleguminous treetamarind

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© Kluwer Academic Publishers 1998