Antonie van Leeuwenhoek

, Volume 73, Issue 2, pp 199–205

Correlation between the regulation of sterigmatocystin biosynthesis and asexual and sexual sporulation in Emericella nidulans

  • Doralinda Guzmán-de-Peña
  • Jesús Aguirre
  • José Ruiz-Herrera
Article

DOI: 10.1023/A:1000820221945

Cite this article as:
Guzmán-de-Peña, D., Aguirre, J. & Ruiz-Herrera, J. Antonie Van Leeuwenhoek (1998) 73: 199. doi:10.1023/A:1000820221945

Abstract

We analyzed the regulation of sterigmatocystin biosynthesis in wild type and mutant strains of Emericella nidulans (= Aspergillus nidulans). A positive correlation between both asexual and sexual sporulation and synthesis of the mycotoxin was observed. Those conditions which favored sporulation stimulated sterigmatocystin formation, and vice versa. Both processes were stimulated by light in a + genetic background. In contrast, they were inhibited by diaminobutanone, an inhibitor of ornithine decarboxylase. The effect of this inhibitor was partially reverted by putrescine addition. Partial supplementation of specific requirements to auxotrophic mutants allowed normal vegetative growth, but interfered with asexual sporulation and sterigmatocystin biosynthesis. Synthesis of the mycotoxin was neither affected in a brlA mutant or in developmental mutants blocked at later steps in sporulation. As in wild type strain, diaminobutanone inhibited sterigmatocystin biosynthesis and cleisthotecia formation in the brlA mutant, and its effect was reverted by addition of putrescine. The inhibitor also affected the transcription of brlA. Our results indicate that sporulation and the synthesis of sterigmatocystin are co-regulated at a step previous to the brlA execution point.

Emericella nidulansAspergillus nidulanssterigmatocystinmycotoxinssporulationpolyaminesdiaminobutanonebrlA

Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Doralinda Guzmán-de-Peña
    • 1
    • 2
  • Jesús Aguirre
    • 3
    • 4
  • José Ruiz-Herrera
    • 1
    • 2
  1. 1.Departamentos de Genética y Biología MolecularUnidad México, D.F.
  2. 2.Ingeniería Genética (Unidad Irapuato)Centro de Investigación y de Estudios Avanzados del IPNIrapuato, GtoMéxico
  3. 3.Instituto de Fisiología CelularUniversidad Nacional Autónoma de MéxicoMexico, D.F.
  4. 4.Departamento de Ingeniería GenéticaCentro de Investigación y de Estudios Avanzados del IPNIrapuato, GtoMexico