Antonie van Leeuwenhoek

, Volume 73, Issue 1, pp 3–8

Influence of plant genotype on the selection of nodulating Sinorhizobium meliloti strains by Medicago sativa

Authors

  • Donatella Paffetti
    • Dipartimento di OrtoflorofrutticolturaUniversità degli Studi di Firenze
  • Fabrice Daguin
    • Dipartimento di Biologia Animale e GeneticaUniversità degli Studi di Firenze
  • Silvia Fancelli
    • Dipartimento di Biologia Animale e GeneticaUniversità degli Studi di Firenze
  • Stefano Gnocchi
    • Istituto Sperimentale Colture Foraggere (I.S.C.F.)
  • Francesca Lippi
    • Dipartimento di Biologia Animale e GeneticaUniversità degli Studi di Firenze
  • Carla Scotti
    • Istituto Sperimentale Colture Foraggere (I.S.C.F.)
  • Marco Bazzicalupo
    • Dipartimento di Biologia Animale e GeneticaUniversità degli Studi di Firenze
Article

DOI: 10.1023/A:1000591719287

Cite this article as:
Paffetti, D., Daguin, F., Fancelli, S. et al. Antonie Van Leeuwenhoek (1998) 73: 3. doi:10.1023/A:1000591719287

Abstract

We analysed the genetic diversity of 270 Sinorhizobium meliloti strains isolated from nodules of three different Medicago sativa varieties, planted in three different Italian soils, combining the Analysis of Molecular Variance (AMOVA) with the Random Amplified Polymorphic DNA (RAPD) technique to estimate variance among RAPD patterns with the aim to draw an objective description of the population genetic structure. Results indicated that a general intraspecific genetic diversity was globally distributed among all the population, however a very high level of diversity was found among strains nodulating different Medicago sativa varieties. Moreover the distribution of the RAPD haplotypes among the plant varieties also showed to be non-random. The overall data indicated that the plant genotype is a major factor in shaping the genetic structure of this natural Rhizobium population.

Sinorhizobium meliloti RAPD AMOVA population genetics

Copyright information

© Kluwer Academic Publishers 1998