Abstract
The lipase from Candida rugosa was immobilized on three commercially available macroporous adsorptive resins for kinetic resolution of ibuprofen. One resin, CRB02, increased the enzyme activity by 50% to 0.027 g g−1 min−1. The deactivation constant (0.19 h−1) of the immobilized enzyme was half of that of the native enzyme and the enantioselectivity (E = 29.2) of the immobilized lipase was 2.2 times as much as that of the native lipase for the kinetic resolution of ibuprofen with 1-propanol in isooctane at 30 °C.
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Yu, H., Wu, J. & Ching, C.B. Enhanced activity and enantioselectivity of Candida rugosa lipase immobilized on macroporous adsorptive resins for ibuprofen resolution. Biotechnology Letters 26, 629–633 (2004). https://doi.org/10.1023/B:BILE.0000023020.12576.1e
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DOI: https://doi.org/10.1023/B:BILE.0000023020.12576.1e