Skip to main content
Log in

Cellulose production by Gluconacetobacter hansenii in a medium containing ethanol

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

The addition of 1% (v/v) ethanol to the basal medium inhibited growth of Gluconacetobacter hansenii but decreased the numbers of non-cellulose producing cells. Cellulose production increased 1.7 times to approx. 2.5 g l−1 and showed a pattern of mixed growth-associated production. Microbial cells produced rigid pellicle-type bacterial cellulose as the shell of a large lump of bacterial cellulose like a static culture. The inoculum cultivated for 3 d maintained cellulose production by the fifth batch.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Borzani W, de Souza SJ (1995) Mechanism of the film thickness increasing during the bacterial production of cellulose on nonagitated liquid media. Biotechnol. Lett. 17: 1271–1272.

    Google Scholar 

  • Delmer DP (1999) Cellulose biosynthesis: exciting times for a diffi-cult field of study. Annu. Rev. Plant Physiol. Plant Mol. Biol. 50: 245–276.

    Google Scholar 

  • Jeong YJ, Lee IS (2000) A view of utilizing cellulose produced by Acetobacter bacteria. Food Ind. Nutr. 5: 25–29.

    Google Scholar 

  • Jung JY, Park YH, Park JK (2003) Effects of alcohols on the production of bacterial cellulose. Korean J. Biotechnol. Bioeng. 18: 127–132.

    Google Scholar 

  • Klemm D, Schumann D, Udhard U, Marsch S (2001) Bacterial synthesized cellulose–artficial blood vessels for microsurgery. Prog. Polym. Sci. 26: 1561–1603.

    Google Scholar 

  • Matthysse AG, Thomas DL, White AR (1995) Mechanism of cellulose synthesis in Agrobacterium tumefaciens. J. Bacteriol. 177: 1076–1081.

    Google Scholar 

  • Naritomi T, Kouda T, Yano H, Yoshinaga F (1998) Effect of ethanol on bacterial cellulose production from fructose in continuous culture. J. Ferment. Bioeng. 85: 598–603.

    Google Scholar 

  • Park JK, Park YH, Jung JY (2003) Production of bacterial cellulose by Gluconacetobacter hansenii isolated from rotten apple. Biotechnol. Bioprocess Eng. 8: 83–88.

    Google Scholar 

  • Schramm M, Hestrin S (1954) Factors affecting production of cellulose at the air/liquid interface of a culture of Acetobacter xylinum. J. Gen. Microbiol. 11: 123–129.

    Google Scholar 

  • Son HJ, Lee OM, Kim YG, Park YK, Lee SJ (2000) Characteristics of cellulose production by Acetobacter sp. A9 in static culture. Korean J. Biotechnol. Bioeng. 15: 573–577.

    Google Scholar 

  • Valla S, Kjosbakken J (1981) Cellulose-negative mutants of Acetobacter xylinum. J. Gen. Microbiol. 128: 1401–1408.

    Google Scholar 

  • Yamanaka S, Watanabe K, Kitamura N, Iguchi M, Mitsuhashi S, Nishi Y, Uryu M (1989) The structure and mechanical properties of sheets prepared from bacterial cellulose. J. Mater. Sci. 24: 3141–3145.

    Google Scholar 

  • Yoshino T, Asakura T, Toda K (1996) Cellulose production by Acetobacter pasteurianus on silicone membrane. J. Ferment. Bioeng. 81: 32–36.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Joong Kon Park.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Park, J.K., Jung, J.Y. & Park, Y.H. Cellulose production by Gluconacetobacter hansenii in a medium containing ethanol. Biotechnology Letters 25, 2055–2059 (2003). https://doi.org/10.1023/B:BILE.0000007065.63682.18

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/B:BILE.0000007065.63682.18

Navigation