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Immunohistochemical localization of human protein 1 in the female prostate (Skene's gland) and the male prostate

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Abstract

Mouse monoclonal anti-urine protein 1 antibody and the biotin-streptavid in-peroxidase technique were used for the immunohistochemical demonstration of human protein 1 in prostatic tissue of both sexes. In the female prostate (Skene's gland), like the male prostate, high expression of human protein 1 was observed on the luminal surface and in the apical cytoplasm of secretory cells of prostatic glands, as well as on the luminal surface of the epithelium of the large ducts of the female prostate and urethra. Expression was also found in the membranes of secretory and basal cells of the glands, in membranes of the urethral uroepithelium and of the female prostate ducts, in the content of glands and ducts, as well as in vascular endothelium and smooth muscle. Human protein 1 (urine protein 1) expression in the secretory cells of the male and female prostate and its incorporation into the surface of cells lining the lumina of the female urethroprostatic complex is indicative not only of the secretory role of protein 1 but also of its potential protective properties operative in shielding the uroepithelium from the aggressive urinary environment. All genito-urinary tissue, and especially the female prostate, were found to be a potential source of urine protein 1 (human protein 1), refuting the notion held so far that it is exclusively the genito-urinary prostatic tissue of the male that participates in its production. The corresponding immunohistochemical distribution of human protein 1 in the same structures of the male and female prostate provides yet another analogous functional-morphological parameter of prostatic tissue in both sexes and further evidence supporting the non-vestigial concept of the prostate in the female.

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References

  • Dhanireddy, R., Kikukawa, T. & Mukherjee, A.B. (1988) Detection of rabbit uteroglobin-like protein in human neonatal tracheobronchial washings. Biochem. Biophys. Res. Commun. 152, 1447–54.

    Article  PubMed  CAS  Google Scholar 

  • Ishii, S., Itoh, Y., Okutani, R., Kawai, T., Kobayashi, Y., Tokue, A. & Asano, Y. (1994) Sex-associated differences in protein 1 values in urine: immunochemical detection of protein 1 in genital tissues. Eur. J. Clin. Chem. Clin. Biochem. 32, 31–6.

    PubMed  CAS  Google Scholar 

  • Itoh, Y., Ishii, S., Okutani, R., Asano, Y. & Kawai, T. (1993) Protein 1: its purification and application in clinical medicine. J. Clin. Lab. Anal. 7, 394–400.

    Article  PubMed  CAS  Google Scholar 

  • Jackson, P.J., Turner, R., Keen, J.N., Brooksbank, R.A. & Cooper, E.H. (1988) Purification and partial amino acid sequence of human urine protein 1. J. Chromatogr. 452, 359–67.

    Article  PubMed  CAS  Google Scholar 

  • Kastendieck, H. & Altenahr, E. (1976) Cyto-and histomorphogenesis of the prostate carcinoma. A comparative light and electron microscopic study. Virchows Arch. A 370, 207–24.

    CAS  Google Scholar 

  • Kikukawa, T., Cowan, B.D., Tejada, R.I. & Mukherjee, A.B. (1988) Detection of a rabbit uteroglobin-like protein in the human endometrium. J. Clin. Endocrinol. Metab. 67, 315–21.

    Article  PubMed  CAS  Google Scholar 

  • Levin, S.W., Butler, J.D., Schumacher, U.K., Wightman, P.D. & Mukherjee, A.B. (1986) Uteroglobin inhibits phospholipase A2 activity. Life Sci. 38, 1813–9.

    Article  PubMed  CAS  Google Scholar 

  • Mantile, G., Miele, L., Cordella-Miele, E., Singh, G., Katyal, S. & Mukherjee, A.B. (1993) Human Clara cell 10 kDa protein is the counterpart of rabbit uteroglobin. J. Biol. Chem. 268, 20343–51.

    PubMed  CAS  Google Scholar 

  • Manyak, M.J., Kikukawa, T. & Mukherjee, A.B. (1988) Expression of uteroglobin-like protein in human prostate. J. Urol. 140, 176–82.

    PubMed  CAS  Google Scholar 

  • Mickey, D.D., Stone, K.R., Stone, M.P. & Paulson, D.F. (1977) Morphologic and immunologic studies of human prostatic carcinoma. Cancer Treat. Rep. 61, 133–8.

    PubMed  CAS  Google Scholar 

  • Ming Chu, T., Kawinski, E., Hibi, N., Groghan, G., Wiley, J., Killian, C.S. & Corral, D. (1989) Prostate-specific antigen domain of human prostate-specific antigen identified with monoclonal antibodies. J. Urol. 141, 152–6.

    Google Scholar 

  • Mujovic, S., Milicevic, M.M. & Milicevic, Z. (1983) Secretory granules in the cells of the rat endothelium. Pathology 11, 170–1.

    Google Scholar 

  • Mukherjee, A.B., Ulane, R.E. & Agrawai, A.K. (1982) Role of uteroglobin and transglutaminase in masking the antigenicity in the rabbit embryos. Am. J. Reprod. Immunol. 2, 135–41.

    PubMed  CAS  Google Scholar 

  • Mukherjee, D.C., Ulane, R.E., Manjunath, R. & Mukherjee, A.B. (1983) Suppression of epididymal sperm antigenicity in the rabbit by uteroglobin and transglutaminase in vitro. Science 219, 989–91.

    Article  PubMed  CAS  Google Scholar 

  • Okutani, R., Itoh, Y., Hirata, H., Kasahara, T., Mukaida, N. & Kawai, T. (1992) Simple and high-yield purification of urine protein 1 using immunoaffinity chromatography: evidence for the identity of urine protein 1 and human Clara cell 10-kilodalton protein. J. Chromatogr. 577, 25–35.

    Article  PubMed  CAS  Google Scholar 

  • Pollen, J.J. & Dreillinger, A. (1984) Immunohistochemical identification of prostatic acid phosphatase and prostate specific antigen in female periurethral glands. Urology 23, 303–4.

    Article  PubMed  CAS  Google Scholar 

  • Sinha, A.A. & Blackhard, C.E. (1973) Ultrastructure of prostatic benign hyperplasia and carcinoma. Urology 2, 114–20.

    Article  PubMed  CAS  Google Scholar 

  • Sloboda, J., Zaviacic, M., Jakubovsky, J., Hammar, E. & Johnsen, J. (1996) Metastasizing adenocarcinoma of the female prostate (Skene's paraurethral glands). Histological and immunohistochemical prostate markers studies and first ultrastructural observation. Path. Res. Pract. (submitted for publication).

  • Svanholm, H., Anderson, O.P. & Rohl, H. (1987) Tumor of female paraurethral duct: immunohistochemical similarity with prostatic carcinoma. Virchows Arch. A 411, 395–8.

    Article  CAS  Google Scholar 

  • Tannenbaum, M. (1983) Prostate cancer grading: light and electron microscopy. Semin. Urol. 1866–92.

  • Tepper, S.L., Jagirdar, J., Heath, D. & Geller, S.A. (1984) Homology between the female paraurethral (Skene's) glands and the prostate. Arch. Pathol. Lab. Med. 108, 423–5.

    PubMed  CAS  Google Scholar 

  • Vasanthakumar, G., Manjunath, R., Mukherjee, A.B., Warabl, H. & Schifman, E. (1988) Inhibition of phagocyte chemotaxis by uteroglobin, an inhibitor of blastocyst rejection. Biochem. Pharmacol. 37, 389–94.

    Article  PubMed  CAS  Google Scholar 

  • Wang, M.C., Valenzuela, L.A., Murphy, G.P. & Chu, T.M. (1979) Purification of a human prostate-specific antigen. Invest. Urol. 17, 159–64.

    PubMed  CAS  Google Scholar 

  • Zaviacic, M. (1984a) Enzyme histochemistry of the adult human female prostate: acid phosphatase distribution. Cell. Mol. Biol. 30, 545–51.

    PubMed  CAS  Google Scholar 

  • Zaviacic, M. (1984b) Enzyme histochemistry of the adult human female prostate: hydrolase and dehydrogenase distribution. Cell. Mol. Biol. 30, 537–43.

    PubMed  CAS  Google Scholar 

  • Zaviacic, M. (1985) The adult human female prostate homologue and the male prostate gland: a comparative enzyme histochemical study. Acta Histochem. 77, 19–31.

    PubMed  CAS  Google Scholar 

  • Zaviacic, M. (1987) The female prostate: nonvestigial organ of the female. A reappraisal (letter). J. Sex Marit. Ther. 13, 148–52.

    CAS  Google Scholar 

  • Zaviacic, M., Brozman, M., HolomÁn, I.K., ZaviacicovÁ, A. & OberucovÁ, J. (1983) New information on the paraurethral (Skene's) ducts and glands in the female (in Slovak, summary in English). Bratisl. Lek. Listy 79, 533–44.

    PubMed  CAS  Google Scholar 

  • Zaviacic, M., ZaviacicovÁ, A., Brozman, M., HolomÁn, I.K., BruchÁc, D., OberucovÁ, J. & Kokavec, M. (1985a) The female prostate or Skene's paraurethral glands and ducts? Reasons for returning to the original term of de Graaf (in Slovak). Cs. Gynek. 50, 372–7.

    CAS  Google Scholar 

  • Zaviacic, M., Brozman, M., ZajÍckovÁ, M., BlazekovÁ, J. & OberucovÁ, J. (1985b) The adult human female urethra. Enzyme-histochemical study. Acta Histochem. 77, 165–75.

    PubMed  CAS  Google Scholar 

  • Zaviacic, M., RuzickovÁ, M., Jakubovsky, J., Danihel, L., BabÁl, P. & BlazekovÁ, J. (1994) The meaning of prostatic markers in orthology of female prostate (in Slovak, summary in English). Bratisl. Lek. Listy 95, 491–7.

    PubMed  CAS  Google Scholar 

  • Zaviacic, M., Sidlo, J. & Borovsky, M. (1993) Prostate specific antigen and prostate specific acid phosphatase in adenocarcinoma of Skene's paraurethral glands and ducts. Virchows Arch. A Pathol. Anat. 423, 503–5.

    Article  CAS  Google Scholar 

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Zaviacic, M., Danihel, L., Ruzickova, M. et al. Immunohistochemical localization of human protein 1 in the female prostate (Skene's gland) and the male prostate. J Mol Hist 29, 219–227 (1997). https://doi.org/10.1023/A:1026401909678

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