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Genetic variability in Turkmen populations of Pistacia vera L.

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Abstract

Seedlings of Pistacia vera L. developed from seeds of two separate populations in Turkmenistan, Kepele and Agachli, were evaluated for their growth potential and genetic polymorphism. Plant growth rate as well as random amplified polymorphic DNA (RAPD) analysis showed distinct differences between the two populations. In plant height growth rate, 17 Agachli accessions were 1.3 times higher on average than that of 10 accessions of Kepele (significant at p = 0.046) and 1.2 times higher for trunk diameter growth rate (p = 0.062). Cluster analysis divided most accessions into two main genetic groups according to their geographic origin. The Agachli group was further divided into two subgroups. One Kepele accession (K4), was genetically different from the rest and clustered on a separate outgroup. Two Agachli accessions (A12 and A17) were outside the two main populations clusters. Accessions K9 and K10 from Kepele were exceptional and were clustered in each of the two Agachli's subgroups, indicating a close genetic relationship between the two populations. In addition, high similarity values (0.58–1.00) and small genetic distances reflect plausible gene flow between Kepele and Agachli, which are 100 Km apart. Mantel test revealed significant relationship between the RAPD and the morphological traits matrices, pointing to the genetic basis for the measured differences in the growth rate. Growing the accessions on the same plot, under similar conditions enabled the evaluation of genotypic differences. The combination of morphological traits and molecular markers will further assist in preservation of genetic variability and cultivation of useful genotypes of P. vera L.

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References

  • Bahrman N., Le Gouis J., Hariri D., Guilbaud L. and Jestin L. 1999. Genetic diversity of old French six-rowed winter barley varieties assessed with molecular, biochemical and morphologi-cal markers and its relation to BaMMV resistance. Heredity 83: 568-574.

    Google Scholar 

  • Doyle J.J. and Doyle J.L. 1987. A rapid isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19: 11-15.

    Google Scholar 

  • Golan-Goldhirsh A. and Kostiukovsky V. 1998. Mediterranean Pistacia genus germplasm collection at Sede Boker Israel. Acta Hort. 470: 131-137.

    Google Scholar 

  • Harrison R.E., Luby J.J., Furnier G.R. and Hancock J.F. 2000. Differences in the apportionment of molecular and morphological variation in North American strawberry and the consequences for genetic resource management. Gen. Res. Crop Evol. 47: 647-657.

    Google Scholar 

  • Hormaza J.I., Dollo L. and Polito V.S. 1994. Determination of relatedness and geographical movements of Pistacia vera L. (pistachio; Anacardiaceae) germplasm by RAPD analysis. Econ. Bot. 48: 349-358.

    Google Scholar 

  • Hormaza J.I., Pinney K. and Polito V.S. 1998. Genetic diversity of pistachio (Pistacia vera, Anacardiaceae) germplasm based on Randomly Amplified Polymorphic DNA (RAPD) markers. Econ. Bot. 52: 78-87.

    Google Scholar 

  • Parfitt D.E. and Badenes M.L. 1997. Phylogeny of the genus Pistacia as determined from analysis of the chloroplast genome. Proc. Natl. Acad. Sci. USA 94: 7987-7992.

    Google Scholar 

  • Popov K.P. 1994. Trees, shrubs, and semishrubs in the mountains of Turkmenistan. In: Fet V. and Atamuradov K.I. (eds), Biogeography and Ecology of Turkmenistan. Kluwer Academic Publishers, pp. 173-186.

  • Rohlf F.J. 1998. NTSYS-pc numerical taxonomy and multivariate analysis system, version 2.0. Exeter Publishing, Ltd., Setauket, New York.

    Google Scholar 

  • Nei M. 1987. Molecular Evolutionary Genetics. Columbia Uni-versity Press, New York.

    Google Scholar 

  • Schoen D.J. and Brown A.H.D. 1993. Conservation of allelic richness in wild crop relatives is aided by assessment of genetic markers. Proc. Natl. Acad. Sci. USA 90: 10623-10627.

    Google Scholar 

  • Zohary M. 1952. A monographical study of the genus Pistacia. Palestine J. Bot. Jerusalem Ser. 5: 187-228.

    Google Scholar 

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Authors and Affiliations

  1. Desert Plant Biotechnology Laboratory, Albert Katz Department of Dryland Biotechnologies, The Jacob Blaustein Institute for Desert Research, Ben Gurion University of the Negev, Sede Boker Campus, 84990, Israel

    Oz Barazani, Bakhtiyor Yakubov, Vladimir Kostiukovsky & Avi Golan-Goldhirsh

  2. National Institute of Deserts, Flora and Fauna, Ministry of Nature Protection of Turkmenistan, Gogol Str. 15, Ashgabat, 744000, Turkmenistan

    Azat Atayev & Konstantin Popov

Authors
  1. Oz Barazani
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  2. Azat Atayev
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  3. Bakhtiyor Yakubov
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  4. Vladimir Kostiukovsky
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  5. Konstantin Popov
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  6. Avi Golan-Goldhirsh
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Barazani, O., Atayev, A., Yakubov, B. et al. Genetic variability in Turkmen populations of Pistacia vera L.. Genetic Resources and Crop Evolution 50, 383–389 (2003). https://doi.org/10.1023/A:1023928017410

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  • DOI: https://doi.org/10.1023/A:1023928017410

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