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Gene Expression Profiling with DNA Microarrays: Advancing Our Understanding of Psychiatric Disorders

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Abstract

DNA microarray transcriptome profiling of the postmortem brain opens novel horizons in understanding molecular changes associated with complex psychiatric disorders. With careful analysis and interpretation of microarray data we are uncovering previously unknown, expression patterns that maybe subject-specific and pivotal in understanding the disease process. In our recent studies, analyses of the prefrontal cortex of subjects with schizophrenia and matched controls uncovered complex changes in the expression of genes related to presynaptic secretory release, GABAergic and glutamatergic transmission, metabolic pathways, myelination, as well as cAMP and phosphoinositol second messenger systems. Our goal will be to integrate this expression data within the context of the relevant anatomical, biochemical, molecular, imaging and clinical findings.

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Authors and Affiliations

  1. Department of Psychiatry, University of Pittsburgh School of Medicine, Pittsburgh, PA, 15261

    Julie Pongrac, David A. Lewis, Pat Levitt & Károly Mirnics

  2. Department of Neurobiology, University of Pittsburgh School of Medicine, Pittsburgh, PA, 15261

    Frank A. Middleton, Pat Levitt & Károly Mirnics

  3. Department of Neuroscience and Physiology, Upstate Medical University, Syracuse, NY, 13210

    Frank A. Middleton

  4. Department of Neuroscience, University of Pittsburgh School of Medicine, Pittsburgh, PA, 15261

    David A. Lewis

  5. John F. Kennedy Center for Human Development, Vanderbilt University, Nashville, TN, 37203

    Pat Levitt

  6. Department of PittArray Core, University of Pittsburgh School of Medicine, Pittsburgh, PA, 15261

    Károly Mirnics

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Pongrac, J., Middleton, F.A., Lewis, D.A. et al. Gene Expression Profiling with DNA Microarrays: Advancing Our Understanding of Psychiatric Disorders. Neurochem Res 27, 1049–1063 (2002). https://doi.org/10.1023/A:1020904821237

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