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Temporal and Spatial Gene Expression of Major Bone Extracellular Matrix Molecules During Embryonic Mandibular Osteogenesis in Rats

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Abstract

It is not known how gene expression of bone extracellular matrix molecules is controlled temporally and spatially, or how it is related with morphological differentiation of osteoblasts during embryonic osteogenesis in vivo. The present study was designed to examine gene expressions of type I collagen, osteonectin, bone sialoprotein, osteopontin, and osteocalcin during mandibular osteogenesis using in situ hybridization. Wistar rat embryos 13–20 days post coitum were used. The condensation of mesenchymal cells was formed in 14-day rat embryonic mandibles and expressed genes of pro-α(I) collagen, osteonectin, bone sialoprotein and osteopontin. Cuboidal osteoblasts surrounding the uncalcified bone matrix were seen as early as in 15-day embryonic mandibles, while flat osteoblasts lining the surface of the calcified bone were seen from 16-day embryonic mandibles. Cuboidal osteoblasts expressed pro-α1(I) collagen, osteonectin and bone sialoprotein intensely but osteopontin very weakly. In contrast, flat osteoblasts expressed osteopontin very strongly. Osteocytes expressed the extracellular matrix molecules actively, in particular, osteopontin. The present study demonstrated the distinct gene expression pattern of type I collagen, osteonectin, bone sialoprotein, osteopontin and osteocalcin during embryonic mandibular osteogenesis in vivo.

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Authors and Affiliations

  1. Division of Oral Molecular Biology, Tohoku University Graduate School of Dentistry, Sendai, 980-8575, Japan

    Jing-Xu Zhu & Manabu Kagayama

  2. Division of Oral Molecular Biology, Tohoku University Graduate School of Dentistry, Sendai, 980-8575, Japan

    Yasuyuki Sasano

  3. Division of Orthodontics, Tohoku University Graduate School of Dentistry, Sendai, 980-8575, Japan

    Ichiro Takahashi

  4. Department of Orthodontics, School of Dentistry, Health Sciences University of Hokkaido, Tobetsu-Ishikari, Hokkaido, 061-0293, Japan

    Itaru Mizoguchi

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  1. Jing-Xu Zhu
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  2. Yasuyuki Sasano
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  3. Ichiro Takahashi
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  4. Itaru Mizoguchi
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  5. Manabu Kagayama
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Zhu, JX., Sasano, Y., Takahashi, I. et al. Temporal and Spatial Gene Expression of Major Bone Extracellular Matrix Molecules During Embryonic Mandibular Osteogenesis in Rats. Histochem J 33, 25–35 (2001). https://doi.org/10.1023/A:1017587712914

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