Abstract
Two full-length cDNAs encoding putative plant phosphate (Pi) transporters were isolated from proteoid roots of Pi-starved white lupin plants 7 and 10 days after emergence (DAE). The deduced amino acid sequences of LaPT1 and LaPT2 are 75.7% identical and both display the typical 12 membrane spanning domains characteristic of other plant Pi transporters. The LaPT1 transcript was expressed most strongly in −P roots (both normal and proteoid) and less so in −P stems and leaves. Transcripts of LaPT1 were low to nondetectable in +P plants. The LaPT2 transcript was highly expressed in roots in both +P and −P treatments. LaPT1 is much more dramatically induced by P deficiency over time, and is more highly expressed in −P proteoid than −P normal root tissue. In contrast, LaPT2 expression was fairly similar over time and in +P and −P normal and −P proteoid roots. While LaPT1 was expressed only under P deficiency, LaPT2 was uniformly expressed by plants exposed to excess Al and deficiencies of N, Mn, Fe and P. The LaPT1 gene was isolated and shown to have two exons interrupted by one intron. The sequence of the 5′-upstream putative promoter was determined.
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References
Braum S M and Helmke P A 1995 White lupin utilizes soil phosphorus that is unavailable to soybean. Plant Soil 176, 95–100.
Bun-ya M, Nishimura M, Harashima S and Oshima Y 1991 The PHO84 gene of Saccharomyces cerevisiae encodes an inorganic phosphate transporter. Mol. Cell. Biol. 11, 3229–3238.
Burleigh S H and Harrison M J 1999 The down regulation of Mt4-like genes by phosphate fertilization occurs systemically and involves phosphate translocation to the shoots. Plant Physiol. 119, 241–248.
Daram P, Brunner S, Rausch C, Steiner C, Amrhein N and Bucher M 1999 Pht2;1 encodes a low-affinity phosphate transporter from Arabidopsis. Plant Cell 11, 2153–2166.
Devereux J, Haeberli P and Smithies O 1984 Sequence analysis programs for the VAX. Nucleic Acids Res. 12, 387–395.
Dinkelaker B, Romheld V and Marschner H 1989 Citric acid excretion and precipitation of calcium citrate in the rhizosphere of white lupin (Lupinus albus L.). Plant Cell Environ. 12, 285–292.
Furihata T, Suzuki M and Sakurai H 1992 Kinetic characterization of two phosphatee uptake systems with different affinities in suspension-cultured Catharanthus roseus protoplasts. Plant Cell Physiol. 33, 1151–1157.
Gardner W K, Parberry D G and Barber D A 1982 The acquisition of phosphorus by Lupinus albus L. I. Some characteristics of the soil/root interface. Plant Soil 68, 19–32.
Gilbert G A, Knight J D, Vance C P and Allan D L 1999 Acid phosphatase activity in phosphorus-deficient white lupin roots. Plant, Cell Environ. 22, 801–810.
Gilbert G A, Knight J D, Vance C P and Allan D L 2000 Proteoid root development of phosphorus deficient lupin is mimicked by auxin and phosphonate. Ann. Bot. 85, 921–928.
Johnson J F, Vance C P and Allan D L 1996 Phosphorus deficiency in Lupinus albus: altered lateral root development and enhanced expression of phosphoenolpyruvate carboxylase. Plant Physiol. 112, 31–41.
Junghans H and Metzlaff M 1990 A simple and rapid method for the preparation of total plant DNA. Biotechniques 8, 176.
Kai M and Adachi H 1999 Phosphate transporter. Published only in DataBase. Accession # of DDBJ/EMBL/GenBank is: ab020061.
Keerthisinghe G, Hocking P J, Ryan P R and Delhaize E 1998 Effect of phosphorus supply on the formation and function of proteoid roots of white lupin (Lupinus albus L.). Plant Cell Environ 21, 467–478.
Lamont B 1982 Mechanisms for enhancing nutrient uptake in plants, with particular reference to Mediterranean South Africa and western Australia. Bot. Rev. 48, 597–689.
Leggewie G, Willmitzer L and Riesmeier J W 1997 Two cDNAs from potato are able to complement a phosphate uptakedeficient yeast mutant: identification of phosphate transporters from higher plants. Plant Cell 9, 381–392.
Liu H, Trieu A T, Blaylock L A and Harrison M J 1998 Cloning and characterization of two phosphate transporters from Medicago truncatula roots: regulation in response to phosphate and to colonization by arbuscular mycorrhizal (AM) fungi. Mol. Plant Microbe Interact. 11, 14–22.
Marschner H 1995 Mineral nutrition in higher plants. Academic Press Inc., San Diego, CA.
Muchhal U S, Pardo J M and Raghothama K G 1996 Phosphate transporters from the higher plant Arabidopsis thaliana. Proc. Natl. Acad. Sci. USA 93, 10519–10523.
Neumann G, Massonneau A, Langlade N, Dinkelaker B, Hengeler C, Romheld V and Martioia E 2000 Physiological aspects of cluster root function and development in phosphorus-deficient white lupin (Lupinus albus L.) Ann. Bot. 85, 909–919.
Raghothama K G 1999 Phosphate acquisition. Annu. Rev. Plant Physiol. Plant Mol. Biol. 50, 665–693.
Sambrook J, Fritsch E and Maniatis T 1989 Molecular cloning. Cold Spring Harbour Laboratory Press, Cold Spring Harbour, NY.
Smith F W, Ealing P M, Dong B and Delhaize E 1997 The cloning of two Arabidopsis genes belonging to a phophate transporter family. Plant J. 11, 83–92.
Rosewarne G M, Barker S J, Smith S E, Smith F A and Schachtman D P 1999 A Lycopersicon esculentum phosphate transporter (LePT1) involved in phosphorus uptake from a vesiculararbuscular mycorrhizal fungus. New Phytol. 144, 507–516.
Vorster P W and Jooste J H 1986 Potassium and phosphate absorption by excised ordinary and proteoid roots of the Protaceae. S. Africa J. Bot. 52, 276–281.
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Liu, J., Uhde-Stone, C., Li, A. et al. A phosphate transporter with enhanced expression in proteoid roots of white lupin (Lupinus albus L.). Plant and Soil 237, 257–266 (2001). https://doi.org/10.1023/A:1013396825577
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DOI: https://doi.org/10.1023/A:1013396825577