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Pinoresinol and matairesinol accumulation in a Forsythia × intermedia cell suspension culture

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Abstract

A newly established Forsythia × intermedia cell suspension culture was shown to accumulate (+)- and (−)-pinoresinol as well as matairesinol. The influence of the sucrose content of the culture medium and of the cultivation time on pinoresinol and matairesinol accumulation was evaluated. The highest pinoresinol yield was achieved from cells grown in medium containing 6% sucrose for 12 ± 2 days with levels of 0.6–0.8 mg g−1 dry weight and an average enantiomeric composition of 75 ± 5% (+)-pinoresinol. The highest matairesinol amount was reached in the same medium at the 14th ± 2 culture day with levels of 1.0–2.7 mg g−1 dry weight. To our knowledge, this is the first report on pinoresinol accumulation in Forsythia × intermedia plants or cell suspension cultures.

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References

  • Borriello SP, Setchell KD, Axelson M & Lawson AM (1985) Production and metabolism of lignans by the human faecal flora. J. Appl. Bact. 58: 37–43

    Google Scholar 

  • Broomhead AJ & Dewick PM (1990) Aryltetralin lignans from Linum flavum and Linum capitatum. Phytochemistry 29: 3839–3844

    Google Scholar 

  • Davin LB, Bedgar DL, Katayama T & Lewis NG (1992) On the stereoselective synthesis of (+)-pinoresinol in Forsythia suspensa from its achiral precursor, coniferyl alcohol. Phytochemistry 31: 3869–3874

    Google Scholar 

  • Davin LB, Wang HB, Crowell AL, Bedgar DL, Martin DM, Sarkanen S & Lewis NG (1997) Stereoselective bimolecular phenoxy radical coupling by an auxiliary (dirigent) protein without an active center. Science 275: 362–366

    Google Scholar 

  • Dewick PM (1994) Forsythia species: In vitro culture and the production of lignans and other secondary metabolites. In: Ba-jaj YPS (ed) Biotechnology in Agriculture and Forestry, Vol 28 Medicinal and Aromatic Plants VI (pp 236–256). Springer, Berlin, Heidelberg

    Google Scholar 

  • Henges A (1999) Biosynthese und Kompartimentierung von Lignanen in Zellkulturen von Linum album. PhD Thesis, Univ. of Düsseldorf, Germany

    Google Scholar 

  • Imbert TF (1998) Discovery of podophyllotoxins. Biochimie 80: 207–222

    Google Scholar 

  • Ingram D, Sanders K, Kolybaba M & Lopez M (1997) Case-control study of phyto-estrogens and breast cancer. Lancet 350: 990–994

    Google Scholar 

  • Lewis NG & Davin LB (1999) Lignans: biosynthesis and function. In: Barton D, Nakanishi K & Meth-Cohn O (eds) Comprehensive Natural Products Chemistry, Vol 1 (pp 639–712). Elsevier, Amsterdam, Lausanne, New York, Oxford, Shannon, Singapore, Tokyo

    Google Scholar 

  • Miyazawa M, Kasahara H & Kameoka H (1992) Phenolic lignans from flower buds of Magnolia fargesii. Phytochemistry 31: 3666–3668

    Google Scholar 

  • Nishibe S, Sakushima A, Kitagawa S, Klimek B, Benecke R & Thieme H (1988) Phenolic compounds from Forsythia leaves. III. On the comparison of constituents betweeen hybrid and parents. Shoyakugaku Zasshi 42: 324–328 (Chemical Abstracts 1989 111: 36661)

    Google Scholar 

  • Okuyama E, Suzumura K & Yamazaki M (1995) Pharmacologically active compounds of Todopon Puok (Fragraea racemosa), a medicinal plant from Borneo. Chem. Pharm. Bull. 43: 2200–2204

    Google Scholar 

  • Petersen M & Alfermann AW (1988) Two new enzymes of rosmarinic acid biosynthesis from cell cultures of Coleus blumei: Hydroxyphenylpyruvate reductase and rosmarinic acid synthase. Z. Naturforsch. 43c: 501–504

    Google Scholar 

  • Petersen M, Strack D & Matern U (1999) Biosynthesis of phenylpropanoids and related compounds. In: Wink M (ed) Annual Plant Reviews Vol 2: Biochemistry of Plant Secondary Metabolism (pp 151–221). Sheffield Academic Press, Sheffield

    Google Scholar 

  • Rahman MMA, Dewick PM, Jackson DE & Lucas JA (1986) Lignans in Forsythia leaves and cell cultures. J. Pharm. Pharmacol. 38: 15 p

    Google Scholar 

  • Rahman MMA, Dewick PM, Jackson DE & Lucas JA (1990a) Lignans of Forsythia intermedia. Phytochemistry 29: 1971–1980

    Google Scholar 

  • Rahman MMA, Dewick PM, Jackson DE & Lucas JA (1990b) Production of lignans in Forsythia intermedia cell cultures. Phytochemistry 29: 1861–1866

    Google Scholar 

  • van Uden W, Pras N & Woerdenbag HJ (1994) Linum species (Flax): in vivo and in vitro accumulation of lignans and other metabolites. In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry, Vol 26. Medicinal and Aromatic Plants VI (pp 219–244). Springer, Berlin, Heidelberg

    Google Scholar 

  • Umezawa T, Davin LB & Lewis NG (1991) Formation of lignans (–)-secoisolariciresinol and (–)-matairesinol with Forsythia intermedia cell free extracts. J. Biol. Chem. 226: 10210–10217

    Google Scholar 

  • Wichers HJ, Harkes MP & Arroo RRJ (1990) Occurrence of 5-methoxypodophyllotoxin in plants, cell cultures and regenerated plants of Linum flavum. Plant Cell Tiss. Org. Cult. 23: 93–100

    Google Scholar 

  • Wichers HJ, Versluis-de Haan GG, Marsman JW & Harkes MP (1991) Podophyllotoxin related lignans in plants and cell cultures of Linum flavum. Phytochemistry 30: 3601–3604

    Google Scholar 

  • Xia ZQ, Costa MA, Proctor J, Davin LB & Lewis NG (2000) Dirigent-mediated podophyllotoxin biosynthesis in Linum flavum and Podophyllum peltatum. Phytochemistry 55: 537–549

    Google Scholar 

Download references

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Correspondence to Maike Petersen.

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Schmitt, J., Petersen, M. Pinoresinol and matairesinol accumulation in a Forsythia × intermedia cell suspension culture. Plant Cell, Tissue and Organ Culture 68, 91–98 (2002). https://doi.org/10.1023/A:1012909131741

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