Abstract
The edible seeds of the quinoa plant contain small quantities of alcohol-soluble protein which, after peptic-tryptic digestion, are unable to agglutinate K562(s) cells. When separated by affinity chromatography on sepharose-6B coupled with mannan, peptic-tryptic digest separated in two fractions. Fraction B peptides (about 1% of total protein) were shown to agglutinate K562(s) cells at a very low concentration, whereas peptides in fraction A and in the mixed fraction A+B were inactive, suggesting that fraction A contains protective peptides that interfere with the agglutinating activity of toxic peptides in fraction B.
Similar content being viewed by others
References
Van de Kramer JH, Weijers HA, Dicke WK (1953) An investigation into the injurious constituents of wheat in connection with their action on patients with celiac disease. Acta Ped 42: 223–231.
Baker PG, Read AE (1976) Oats and barley toxicity in celiac patients. Postgrad Med J 52: 264–268.
Cornell HJ, Townley RW (1973) The toxicity of certain cereal proteins in celiac disease. Gut 15: 223–231.
Strober W (1979) An immunological theory of gluten-sensitive enteropathy. In:McNicholl B, McCarthy CF, Fottrell PF (eds), Perspectives in coeliac disease. Lancaster, MTP Press, pp. 169–174.
Auricchio S, De Ritis G, De Vincenzi M, Minetti M, Sapora O, Silano V (1984) Agglutinating activity of gliadin derived peptides from bread wheat: implications for coeliac disease. Biochem Biophys Res Commun 21: 428–433.
Auricchio S, Arco A, Dauria G, De Ritis G, De Vincenzi M, Magazzu G, Maiuri L, Silano V (1987) A-gliadin related synthetic peptides agglutinate undifferentiated K562(s) cells and affect in vitro developing fetal rat intestine and cultured atrophic coeliac mucosa. Gastroenterology 92: 1301A.
Auricchio S, De Ritis G, De Vincenzi M, Magazzu G, Maiuri L, Mancini E, Sapora O, Silano V (1990) Mannan and oligomers of N-acetylglucosamine protect intestinal mucosa of coeliacs with active disease from in vitro toxicity of gliadin peptides. Gastroenterology 99: 973–978.
Auricchio S, De Ritis G, De Vincenzi M, Gentile V, Maiuri L, Porta R, Raia V (1990) Amines protect in vitro the coeliac small intestine from damaging activity of gliadin peptides. Gastroenterology 99: 1668–1674.
De Ritis G, Auricchio S, De Vincenzi M, Jones HW, Lew EJ, Bernardin JE, Silano V (1987) A-gliadin peptides of known amino acidic sequence: damaging effect on the in vitro cultured atrophic coeliac intestinal mucosa and agglutinating activity on K562(s) cells. Gastroenterology 92: 1368A.
De Vincenzi M, Dessì MR, Giovannini C, Maialetti F, Mancini E (1995) Agglutinating activity of gliadin peptide fractions in coeliac disease. Toxicology 96: 29–35.
Auricchio S, De Ritis G, De Vincenzi M, Maiuri L, Raia V, Silano V (1987) Prevention by mannan and other sugars of in vitro damage of rat fetal small intestine induced by cereal prolamin peptides toxic for human celiac intestine. Ped Res 22: 703–707.
De Ritis G, Occorsio P, Auricchio S, Gramenzi F, Morisi G, Silano V (1979) Toxicity of wheat flour proteins and protein-derived peptides for in vitro developing intestine from rat fetus. Pediatr Res 13: 1255–1261.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with folin phenol reagent. J Biol Chem 193: 265–275.
Lever H (1972) A new reaction for colorimetric determination of carbohydrates. Anal Biochem 47: 279–282.
Skerrit JH, Hill AS (1991) Enzyme immunoassay for determination of gluten in foods: collaborative study. J Assoc Off Anal Chem 74: 257–264.
Carratù B, Boniglia C, Bellomonte G (1995) Optimization of the determination of aminoacids in parenteral solutions by high-performance liquid chromatography with precolumn derivatization using 9-fluorenylmethyl chloroformate. J Chromatogr A 708: 203–208.
Gehrke CW, Wall LL, Absheer JS, Kaiser FE, Zumwalt RW (1985) Focus: amino acid analysis. J Assoc Off Anal Chem 68(5): 811–821.
Mason VC, Bech-Andersen S (1980) Hydrolysate preparation for amino acid determinations in feed constituents. Z Tierphysiol Tierernahrg u Futtermittelkde 43: 146–164.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
De Vincenzi, M., Silano, M., Luchetti, R. et al. Agglutinating activity of alcohol-soluble proteins from quinoa seed flour in celiac disease. Plant Foods Hum Nutr 54, 93–100 (1999). https://doi.org/10.1023/A:1008059519025
Issue Date:
DOI: https://doi.org/10.1023/A:1008059519025