Abstract
During the hunting seasons 1995–96 to 1997–98, 19 red deer from the Upper Susa Valley (Cottian Alps) were examined for paratuberculosis (Johne's disease). Specific DNA amplification on mesenteric lymph nodes detected Mycobacterium avium paratuberculosis in 17 animals. Ten of these red deer were tested for serum antibodies by the AGID and ELISA tests, nine being negative. Three isolates from infected deer were genetically characterized by an arbitrarily primed polymerase chain reaction, and showed similar genetic polymorphism to that of bovine strains isolated in different Italian areas. The study showed that paratuberculosis is present in red deer of the Upper Susa Valley and that serological tests are not an efficient means for monitoring this infection.
Similar content being viewed by others
REFERENCES
Belletti, G.L. and Zavanella, M., 1987. L'esame colturale delle feci per la diagnosi di paratubercolosi. Selezione Veterinaria, 28, 891-896
De Lisle, G.W. and Collins, D.M., 1995. Johne's disease in red deer. The Veterinary Record, 1, 336
Fawcett, A.R., Goddard, P.J., McKelvey, W.A.C., Buxton, D., Reid, H.W., Greig, A. and Macdonald, A.J., 1995. Johne's disease in a herd of farmed red deer. The Veterinary Record, 136, 165-169
François, B., Krishnamoorthy, R. and Elion, J., 1997. Comparative study of Mycobacterium paratuberculosis strains isolated from Crohn's disease and Johne's disease using restriction fragment length polymorphism and arbitrarily primed polymerase chain reaction. Epidemiology and Infection, 118, 227-233
Gennero, M.S., Meneguz, P.G., Mandola, M.L., Masoero, L., De Meneghi, D. and Rossi, L., 1993. Indagini sierologiche su ruminanti selvatici in Piemonte. Società Italiana delle Scienze Veterinarie, 47, 979-983
Green, E.P., Tizard, M.L.V., Moss, M.T., Thompson, J., Winterbourne, D.J., McFadden, J.J. and Hermon-Taylor, J., 1989. Sequence and characteristic of IS900, an insertion element identified in a human Crohn's disease isolate of Mycobacterium paratuberculosis. Nucleic Acids Research, 17, 9063-9087
Ifearulundu, Y.J.J. and Kaneene, J.B., 1997. Epidemiological and economic impact of subclinical Johne's disease: a review. Veterinary Bulletin, 67, 437-447
Maglione, E., Nebbia, P., Robino, P. and Miglietti, E., 1996. Paratubercolosi nel bovino. Large Animal Review, 4, 29-33
Pacetti, A., Belletti, G., Fabbi, M., Mutinelli, F. and Genchi, C., 1994. Paratubercolosi del cervo. Obiettivi e Documenti veterinari, 4, 67-70
Perez, V., Tellechea, J., Corpa, J.M., Gutierrez, M.M., Bolea, R., Badiola, J.J. and Garcia Marin, J.F., 1997. Pathology and diagnostic tests in ovine paratuberculosis. Proceedings of the 15th meeting of the European Society of Veterinary Pathology, 84
Power, S.B., Haagsma, J. and Smyth, D.P., 1993. Paratuberculosis in farmed red deer (Cerva elaphus) in Ireland. The Veterinary Record, 132, 213-216
Ridge, S.E., 1994. Evaluation of an absorbed enzyme-linked immunosorbent assay for bovine Johne's disease, (MVS thesis, University of Melbourne)
Riemann, H., Zaman, M.R., Ruppanner, R., Aalung, O., Jorgensen, J.B., Worsaae, H. and Behymer, D., 1979. Paratuberculosis in cattle and free-living exotic deer. Journal of the American Veterinary Medical Association, 174, 841-843
Rosati, S., Robino, P. and Nebbia, P., 1998. Caratterizzazione genetica di stipiti di Mycobacterium paratuberculosis isolati in Italia. Atti Società Italiana di Buiatria, 30, 369-376
Sherman, D.M., Markham, J.F. and Bates, F., 1984. Agar gel immunodiffusion tests for diagnosis of clinical paratuberculosis in cattle. Journal of the American Veterinary Medical Association, 185, 179-182
Sockett, D.C., Carr, D.J., Richards, W.D. and Collins, M.T., 1992a. A repository of samples for comparison of diagnostic testing procedures for bovine paratuberculosis. Journal of Veterinary Diagnostic Investigation, 4, 188-191
Sockett, D.C., Conrad, T.A., Thomas, C.B. and Collins, M.T., 1992b. Evaluation of four serological tests for bovine paratuberculosis. Journal of Clinical Microbiology, 30, 1134-1139
Stehman, S.M., 1996. Paratuberculosis in small ruminants, deer, and South American camelids in veterinary clinics of North America. Food Animal Practice, 12, 441-455
Thoen, C.O. and Haasma, J., 1996. Molecular techniques in the diagnosis and control of paratuberculosis in cattle. Journal of the American Veterinary Medical Association, 209, 734-737
Thorel, M.F., Kriechevsky, M. and Levy-Frebault, V.V., 1990. Numerical taxonomy of mycobactin-dependent mycobacteria, emended description of Mycobacterium avium, and description of Mycobacterium avium subsp., avium subsp. nov., Mycobacterium avium subsp. paratuberculosis subsp. nov., and Mycobacterium avium subsp. silvaticum subsp. nov. International Journal of Systematic Bacteriology, 40, 254-260
Vary, P.H., Andersen, P.R., Green, E., Hermon-Taylor, J. and McFadden, J.J., 1990. Use of highly specific DNA probes and the polymerase chain reaction to detect Mycobacterium paratuberculosis in Johne's disease. Journal of Clinical Microbiology, 28, 933-937
Yokomizo, Y. and Merkal, R.S., 1983. Enzyme-linked immunosorbent assay for detection of bovine immunoglobulin G1 antibody to a protoplasmic antigen of Mycobacterium paratuberculosis. American Journal of Veterinary Research, 44, 2205-2207
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nebbia, P., Robino, P., Ferroglio, E. et al. Paratuberculosis in Red Deer (Cervus elaphus hippelaphus) in the Western Alps. Vet Res Commun 24, 435–443 (2000). https://doi.org/10.1023/A:1006482520336
Issue Date:
DOI: https://doi.org/10.1023/A:1006482520336