Abstract
The responses of photosynthesis, Rubisco activity, Rubisco protein, leaf carbohydrates and total soluble protein to three carbon dioxide treatments were studied in winter wheat [Triticum aestivum (L.)] and barley [Hordeum vulgare (L.)]. Barley and wheat plants were grown in small field plots during 1995 and 1996 in clear, acrylic chambers (1.2–2.4 m2) and were provided with continuous carbon dioxide fertilization at concentrations of 350, 525 and 700 μmol mol−1. Photosynthetic rates of barley penultimate leaves and wheat flag leaves measured at growth carbon dioxide concentrations decreased with leaf age in all three CO2 treatments during 1995 and 1996. Photosynthetic acclimation to elevated CO2 was observed on seven of eight measurement dates for barley and ten of eleven measurement dates for wheat over both years. Initial Rubisco activity, total soluble protein and Rubisco protein in barley penultimate leaves and wheat flag leaves also decreased with leaf age. Total Rubisco activity was not used because of enzyme degradation. There was a significant CO2 treatment effect on initial Rubisco activity, total soluble protein and Rubisco protein for wheat in 1995 and 1996 and for barley in 1995. Responses of barley penultimate leaf Rubisco activity and leaf protein concentrations to elevated carbon dioxide were nonsignificant in 1996. A significant CO2 treatment effect also was detected when means of Rubisco activity, soluble protein and Rubisco protein for wheat flag leaves were combined over harvests and years. These three flag leaf parameters were not significantly different in the 350 and 525 μmol mol−1 CO2 treatments but were decreased during growth in 700 μmol mol−1 CO2 relative to the other two CO2 treatments. Ratios of photosynthesis at 700 and 350 μmol mol−1 were compared to ratios of Rubisco activity at 700 and 350 μmol mol−1 using wheat flag leaf data from 1995 and 1996. Regression analysis of these data were linear [y = 0.586 + 1.103t x (r2 = 0.432)] and were significant at P ≤ 0.05. This result indicated that photosynthetic acclimation was positively correlated with changes of initial Rubisco activity in wheat flag leaves in response to CO2 enrichment. Effects of elevated CO2 on wheat leaf proteins during 1995 and 1996 and on barley during 1995 were consistent with an acceleration of senescence.
Similar content being viewed by others
References
Austin RB and Edrich J (1975) Effects of ear removal on photosynthesis, carbohydrate accumulation and on the distribution of assimilated 14C in wheat. Ann Bot 39: 141–151
Badger M (1992) Manipulating agricultural plants for a future high CO2 environment. Aust J Bot 40: 421–429
Bowes G (1991) Growth at elevated CO2: Photosynthetic responses mediated through Rubisco. Plant Cell Environ 14: 795–806
Bradford MM (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248–254
Bunce JA (1993) Growth, survival, competition, and canopy carbon dioxide and water vapor exchange of first year alfalfa at an elevated CO2 concentration. Photosynthetica 29: 557–565
Bunce JA (1995) Long-term growth of alfalfa and orchard grass plots at elevated carbon dioxide. J Biogeography 22: 341–348
Bergmeyer HU, Brent E, Schmidt F and Stock H (1974) D-Glucose. Determination with hexokinase and glucose 6-phosphate dehydrogenase. In: Bergmeyer HU (ed) Methods of Enzymatic Analysis, Vol 3, 2nd ed, pp 1196–1198, Academic Press, New York
Campbell WJ, Allen LH, Jr and Bowes g (1988) Effects of CO2 concentration on Rubisco activity, amount, and photosynthesis in soybean leaves. Plant Physiol 67: 1007–1010
Follett RF (1993) Global climate change, US agriculture, and carbon dioxide. J Prod Agric 6: 181–190
Idso S and Kimball BA (1992) Effects of atmospheric CO2 enrichment on photosynthesis, respiration, and growth of sour orange trees. Plant Physiol 99: 341–343
Kimball BA and Mauney JR (1993) Response of cotton to varying CO2, irrigation and nitrogen: yield and growth. Agron J 85: 706–712
Kramer PJ (1981) Carbon dioxide concentration, photosynthesis, and dry matter production. BioScience 31: 29–33
Krapp A, Quick WP, Stitt M (1991) Ribulose-1,5-bisphosphate carboxylase oxygenase, other Calvin cycle enzymes, and chlorophyll decrease when glucose is supplied to mature spinach leaves via the transpiration stream. Planta 186: 58–69
Makino A, Mae T and Ohira K (1986) Colorimetric measurement of proteins stained with Coomassie Brillant Blue R on sodium dode-cylsulfate polyacrylamide gel electrophoresis by eluting with formamide. Agric Biol Chem (Tokyo) 50: 1911–1912
Nie GY, Long SP, Garcia RL, Kimball BA, Lamporte RL, Pinter PJ, Jr, Wall GW and Webber AN (1995) Effects of free-air CO2 enrichment on the development of the photosynthetic apparatus in wheat, as indicated by changes in leaf proteins. Plant Cell Environ 18: 855–864
Perchorowicz JT, Raynes DA and Jensen RG (1981) Light limitation of photosynthesis and activation of ribulose bisphosphate carboxylase in wheat seedlings. Proc Natl Acad Sci (USA) 78: 2985–2989
Rogers GS, Payne L, Milham P and Conroy J (1993) Nitrogen and phosphorous requirements of cotton and wheat under changing CO2 concentrations. Plant and Soil 155/156: 231–234
Sage RF, Sharkey TD and Seemann JR (1989) Acclimation of photosynthesis to elevated CO2 in five C3 species. Plant Physiol 89: 590–596
Sheen J (1990) Metabolic repression of transcription in higher plants. Plant Cell 2: 1027–1038
Sheen J (1994) Feedback control of gene expression. Photosynth Res 39: 427–438
Sicher RC, Kremer DF and Bunce JA (1995) Photosynthetic acclimation and photosynthate partitioning in soybean leaves in response to carbon dioxide enrichment. Photosynth Res 46: 409–417
Sicher RC, Kremer DF and Rodermel SR (1994) Photosynthetic acclimation to elevated CO2 occurs in transformed tobacco with decreased ribulose-1,5-bisphosphate carboxylase/oxygenase content. Plant Physiol 104: 409–415
Sionit N, Hellmers H and Strain BR (1980) Growth and yield of wheat under CO2 enrichment and water stress. Crop Sci 20: 687–690
Stitt M (1991) Rising CO2 levels and their potential significance for carbon flow through photosynthesis. Plant Cell Environ 14: 741–762
Stitt M and Schulze D 1994 Does Rubisco control the rate of photosynthesis and plant growth? An excercise in molecular ecophysiology. Plant Cell Environ 17: 465–487
Webber AN, Nie G-Y and Long SP (1994) Acclimation of photosynthetic proteins to rising atmospheric CO2. Photosynth Res 39: 413–425
Wong SC (1979) Elevated atmospheric partial pressure of CO2 and plant growth. I. Interactions of nitrogen nutrition and photosynthetic capacity of C3 and C4 plants. Oecologia 44: 68–74
Yelle S, Beeson RC, Trudel MJ and Gosselin A (1989) Acclimation of two tomato species to high atmospheric CO2. II. Ribulose 1,5-bisphosphate carboxylase oxygenase and phosphoenolpyruvate carboxylase. Plant Physiol 90: 1473–1477
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sicher, R.C., Bunce, J.A. Relationship of photosynthetic acclimation to changes of Rubisco activity in field-grown winter wheat and barley during growth in elevated carbon dioxide. Photosynthesis Research 52, 27–38 (1997). https://doi.org/10.1023/A:1005874932233
Issue Date:
DOI: https://doi.org/10.1023/A:1005874932233