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Intermittent maltose feeding enhances paclitaxel production in suspension culture of Taxus chinensis cells

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Abstract

High paclitaxel production (26 mg l−1) was achieved through the intermittent feeding of 3, 1, and 2% (w/v) sucrose at days 0, 7, and 21, respectively, to a suspension culture of Taxus chinensis cells. Intermittent feedings of 1 and 2% (w/v) maltose at days 7 and 21, respectively, to the culture increased the paclitaxel production up to 67 mg l−1.

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References

  • Choi HK, Adams TL, Stahlhut W, Kim SI, Yun JH, Song BK, Kim JH, Song JS, Hong SS, Lee HS (1999) Method for mass production of taxol by semi-continuous culture with Taxus chinensis cell culture. US Patent 5,871,979.

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 151-158.

    PubMed  Google Scholar 

  • Hirasuna TJ, Pestchanker LJ, Srinivasan V, Shuler ML (1996) Paclitaxel production in suspension cultures of Taxus baccata. Plant Cell Tiss. Org. Cult. 44: 95–102.

    Google Scholar 

  • Ketchum REB, Gibson DM (1996) Paclitaxel production in suspension cell cultures of Taxus. Plant Cell Tiss. Org. Cult. 46: 9–16.

    Google Scholar 

  • Kim JH, Yun JH, Hwang YS, Byun SY, Kim DI (1995) Production of paclitaxel and related taxanes in Taxus brevifolia cell cultures: effect of sugar. Biotechnol. Lett. 17: 101–106.

    Google Scholar 

  • Koch KE (1996) Carbohydrate-modulated gene expression in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 47: 509–540.

    PubMed  Google Scholar 

  • Suffness M, Wall ME (1995) Discovery and development of taxol. In: Suffness M, ed. Taxol: Science and Application. Boca Raton: CRC Press, pp. 3–25.

    Google Scholar 

  • Wang HQ, Yu JT, Zhong JJ (2000) Significant improvement of taxane production in suspension cultures of Taxus chinensis by sucrose feeding strategy. Process Biochem. 35: 479–483.

    Google Scholar 

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Authors and Affiliations

  1. Samyang Genex Biotech Research Institute, Taejon, 305-348, Korea

    Hyung-Kyoon Choi, Sang-Ic Kim, Joo-Sun Son, Seung-Suh Hong & Hyun-Soo Lee

  2. Department of Genetic Engineering, Kyung Hee University, Suwon, 449-701, Korea

    In-Sik Chung

  3. Department of Food Science and Technology, School of Agricultural Biotechnology, Seoul National University, Suwon, 441-100, Korea

    Hyong-Joo Lee

Authors
  1. Hyung-Kyoon Choi
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  2. Sang-Ic Kim
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  3. Joo-Sun Son
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  4. Seung-Suh Hong
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  5. Hyun-Soo Lee
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  6. In-Sik Chung
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  7. Hyong-Joo Lee
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Choi, HK., Kim, SI., Son, JS. et al. Intermittent maltose feeding enhances paclitaxel production in suspension culture of Taxus chinensis cells. Biotechnology Letters 22, 1793–1796 (2000). https://doi.org/10.1023/A:1005658405449

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  • DOI: https://doi.org/10.1023/A:1005658405449

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