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Enzymatic evaluation of Bacillus amyloliquefaciens phytase as a feed additive

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Abstract

Phytases from Bacillus amyloliquefaciens DS11 and Aspergillus ficuum for feed enzyme were compared on the basis of phosphate inhibition and thermal stabilities. The apparent half-life of the former enzyme at 80 °C was 42 min. The activity of B. amyloliquefaciens phytase was retained up to 5 mM phosphate while 3 mM phosphate inhibited 50% of the original activity in case of A. ficuum phytase. Addition of 5 mM CaCl2 significantly broadened the active pH range and also increased the pH stability of DS11 phytase.

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References

  • Adeola O, Lawrence BV, Sutton AL, Cline TR (1995) Phytase-induced changes in mineral utilization in zinc-supplemented diets for pigs. J. Anim. Sci. 73: 3384–3391.

    Google Scholar 

  • Dressman JB, Berardi RR, Dermentzoglou LC, Russell TL, Schmaltz SP, Barnett JL, Jarvenpaa KM (1990) Upper gastrointestinal (GI) pH in young, healthy men and women. Pharm. Res. 7: 756–761.

    Google Scholar 

  • Engelen AJ, van der Heeft FC, Randsdorp PH, Smit EL (1994) Simple and rapid determination of phytase activity. J. AOAC Int. 77: 760–764.

    Google Scholar 

  • Kim YO, Lee JK, Kim HK, Yu JH, Oh TK (1998) Cloning of the thermostable phytase gene (phy) from Bacillus sp. DS11 and its overexpression in Escherichia coli. FEMS Microbiol. Lett. 162: 185–191.

    Google Scholar 

  • Lei XG, Ku PK, Miller ER, Yokoyama MT, Ullrey DE (1994) Ca2+ level affects the efficacy of supplemental microbial phytase in corn-soybean meal diets of weanling pigs. J. Anim. Sci. 72: 139–143.

    Google Scholar 

  • Maenz DD, Classen HL (1998) Phytase activity in the small intestinal brush border membrane of the chicken. Poult. Sci. 77: 557–563.

    Google Scholar 

  • Pallauf J, Rimbach G (1997) Nutritional significance of phytic acid and phytase. Arch. Tierernahr. 50: 301–319.

    Google Scholar 

  • Wyss M, Pasamontes L, Remy R, Kohler J, Kusznir E, Gadient M, Muller F, van Loon A (1998) Comparison of the thermostability properties of three acid phosphatases from molds: Aspergillus fumigatus phytase, A. niger phytase, and A. niger pH 2.5 acid phosphatase. Appl. Environ. Microbiol. 64: 4446–4451.

    Google Scholar 

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Authors and Affiliations

  1. Korea Research Institute of Bioscience and Biotechnology, Yusong, Taejon, 305-600, Korea

    Dong-Hyun Kim, Byung-Chul Oh, Won-Chan Choi, Jung-Kee Lee & Tae-Kwang Oh

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  1. Dong-Hyun Kim
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  2. Byung-Chul Oh
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  3. Won-Chan Choi
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  4. Jung-Kee Lee
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  5. Tae-Kwang Oh
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Kim, DH., Oh, BC., Choi, WC. et al. Enzymatic evaluation of Bacillus amyloliquefaciens phytase as a feed additive. Biotechnology Letters 21, 925–927 (1999). https://doi.org/10.1023/A:1005602717835

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  • DOI: https://doi.org/10.1023/A:1005602717835

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