Abstract
Cuticular hydrocarbons appear to play a role in ant nestmate recognition, but few studies have tested this hypothesis experimentally with purified hydrocarbon extracts. We exposed captive colonies of the harvester ant Pogonomyrmex barbatus to small glass blocks coated with whole cuticular lipid extracts and the purified hydrocarbon portion of extracts from nestmate and nonnestmate workers. As an estimate of agonistic behavior, we measured the proportion of ants in contact with blocks that flared their mandibles. Blocks coated with cuticular extracts from nonnestmates were contacted by more workers in one of two experiments and elicited higher levels of aggression in both experiments than blocks bearing extracts from nestmates. The cuticular hydrocarbon fraction of extracts alone was sufficient to elicit agonistic behavior toward nonnestmates. The results demonstrate that harvester ants can perceive differences in cuticular hydrocarbon composition, and can use those differences in nestmate recognition.
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REFERENCES
Bonavita-Cougourdan, A., Lement, J. L., and Lange, C. 1987. Nestmate recognition: The role of cuticular hydrocarbons in the ant Camponotus vagus scop. J. Entomol. Sci. 22:1-10.
Brown, M. J. F., and Gordon, D. M. 1997. Individual specialisation and encounters between harvester ant colonies. Behaviour 134:849-866.
Dahbi, A., Cerda, X., Hefetz, A., and Lenoir, A. 1996. Social closure, aggressive behavior, and cuticular hydrocarbon profiles in the polydomous ant Catagluphis iberica (Hymenoptera, Formicidae). J. Chem. Ecol. 22:2173-2186.
Espelie, K. E., and Bernays, E. A. 1989. Diet-related differences in the cuticular lipids of Manduca sexta larvae. J. Chem. Ecol. 15:2003-2017.
Gamboa, G. J., Grudzien, T. A., Espelie, K. E., and Bura, E. A. 1996. Kin recognition pheromones in social wasps: Combining chemical and behavioural evidence. Anim. Behav. 51:625-629.
Gordon, D. M. 1986. The dynamics of the daily round of the harvester ant colony (Pogonomyrmex barbatus). Anim. Behav. 34:1402-1419.
Gordon, D. M., and Kulig, A. W. 1996. Founding, foraging, and fighting: Colony size and the spatial distribution of harvester ant nests. Ecology 77:2393-2409.
Gordon, D. M., and Mehdiabadi, N. J. 1999. Encounter rate and task allocation in harvester ants. Behav. Ecol. Sociobiol. 45:370-377.
Howard, R. W. 1993. Cuticular hydrocarbons and chemical communication, pp. 179-226, in W. Stanley-Samuelson and D. R. Nelson (ed.). Insect Lipids: Chemistry, Biochemistry and Biology, University of Nebraska Press, Lincoln.
Jackson, L. L., Armold, M. T., and Regnier, R. E. 1974. Cuticular lipids of adult fleshflies, Sarcophaga bullata. Insect Biochem. 4:369-379.
Jutsum, A. R., Saunders, T. S., and Cherrett, J. M. 1979. Intraspecific aggression in the leafcuting ant Acromyrmex octospinosus. Anim. Behav. 27:839-844.
Lahav, S., Soroker, V., and Hefetz, A. 1999. Direct behavioral evidence for hydrocarbons as ant recognition discriminators. Naturwissenschaften 86:246-249.
Le Moli, F., and Mori, Mori 1990. Laboratory experiments on environmental sources of nestmate and nonnestmate discrimination in three species of Formica ants (Hymenoptera: Formicidae). Psyche 97:147-169.
Liu, Z., Yamane, S., Wang, Q., and Yamamoto, H. 1998. Nestmate recognition and temporal modulation in the patterns of cuticular hydrocarbons in natural colonies of Japanese carpenter ants, Camponotus japonicus Mayr (Hymenoptera: Formicidae). J. Ethol. 16:57-65.
Lockey, K. H. 1988. Lipids of the insect cuticle: Origin, composition and function. Comp. Biochem. Physiol. 89B:595-645.
Nelson, D. R., Fatland, C. L., Howard, R. W., Mcdaniel, C. A., and Blomquist, G. J. 1980. Re-analysis of the cuticular methylalkanes of Solenopsis invicta and S. richteri. Insect Biochem. 10:409-418.
Nowbahari, E., Lenoir, A., ClÉment, J. L., Lange, C., BagnÈres, A. G., and Joulie, C. 1990. Individual, geographical and experimental variation of cuticular hydrocarbons of the ant Cataglyphis cursor (Hymenoptera: Formicidae): their use in nest and subspecies recognition. Biochem. Syst. Ecol. 18:63-73.
Nowbahari, M., and Lenoir, A. 1984. Le fermeture des sociétés de la fourmi Cataglyphis cursor: Relations avec la distance géographique, pp. 457-461, in A. De Haro and X. Espadaler (eds.). Processus d'acquisition précoce. Les communications. Universitat Autonoma de Barcelona, Barcelona.
Obin, M. S. 1986. Nestmate recognition cues in laboratory and field colonies of Solenopsis invicta Buren (Hymenoptera: Formicidae): Effect of environment and role of cuticular hydrocarbons. J. Chem. Ecol. 12:1965-1975.
Page, M., Nelson, L. J., Haverty, M. I., and Blomquist, G. J. 1990. Cuticular hydrocarbons of eight species of North American cone beetles, Conophthorus Hopkins. J. Chem. Ecol. 16:1173-1198.
Panel on Discriminant Analysis and Clustering. 1989. Discriminant analysis and clustering. Stat. Sci. 4:34-69.
Provost, E., RiviÈre, G., Roux, M., Morgan, E. D., and BagnÈres, A. G. 1993. Change in the chemical signature of the ant Leptothorax lichtensteini Bondroit with time. Insect Biochem. Mol. Biol. 23:945-957.
Singer, T. L. 1998. Roles of hydrocarbons in the recognition systems of insects. Am. Zool. 38:394-405.
Singer, T. L., and Espelie, K. E. 1992. Social wasps use nest paper hydrocarbons for nestmate recognition. Anim. Behav. 44:63-68.
Singer, T. L., and Espelie, K. E. 1996. Nest surface hydrocarbons facilitate nestmate recognition for the social wasp, Polistes metricus Say (Hymenoptera, Vespidae). J. Insect Behav. 9:857-870.
Smith, B. H., and Breed, M. D. 1995. The chemical basis for nestmate recognition and mate discrimination in insects, pp. 287-317, in R. T. Cardé and W. J. Bell (eds.). Chemical Ecology of Insects 2. Chapman & Hall, New York.
Sokal, R. R., and Rohlf, F. J. 1994. Biometry, 3rd ed. W. H. Freeman & Co., New York.
Thomas, M. L., Parry, L. J., Allan, R. A., and Elgar, M. A. 1999. Geographic affinity, cuticular hydrocarbons and colony recognition in the Australian meat ant Iridomyrmex purpureus. Naturwissenschaften 86:87-92.
Vander Meer, R. K., and Morel, L. 1998. Nestmate recognition in ants, pp. 79-103, in R. K. Vander Meer, M. D. Breed, K. E. Espelie, and M. L. Winston (ed.). Pheromone Communication in Social Insects. Westview Press, Boulder, Colorado.
Vander Meer, R. K., Saliwanchik, D., and Lavine, B. 1989. Temporal changes in colony cuticular hydrocarbon patterns of Solenopsis invicta: Implications for nestmate recognition. J. Chem. Ecol. 15:2115-2125.
Wagner, D., Brown, M. J. F., Broun, P., Cuevas, W., Moses, L. E., Chao, D. L., and Gordon, D. M. 1998. Task-related differences in the cuticular hydrocarbon composition of harvester ants, Pogonomyrmex barbatus. J. Chem. Ecol. 24:2021-2037.
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Wagner, D., Tissot, M., Cuevas, W. et al. Harvester Ants Utilize Cuticular Hydrocarbons in Nestmate Recognition. J Chem Ecol 26, 2245–2257 (2000). https://doi.org/10.1023/A:1005529224856
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DOI: https://doi.org/10.1023/A:1005529224856