Abstract
In animals, V-ATPases are believed to play roles in the plasma membrane,as well as endomembrane. To understand these different functions, it isnecessary to adopt a genetic approach in a physiologically tractable modelorganism. For this purpose, Drosophila melanogaster is ideal,because of the powerful genetics associated with the organism and because ofthe unusually informative epithelial phenotype provided by the Malpighiantubule. Recently, the first animal “knockouts” of a V-ATPase weredescribed in Drosophila. The resulting phenotypes have generalutility for our understanding of V-ATPase function and suggest a screen fornovel subunits and associated proteins. Genome project resources haveaccelerated our knowledge of the V-ATPase gene family size and the newDrosophila genes vhaSFD, vha100-1, vha100-2, vha100-3, vha16-2,vha16-3, vha16-4, vhaPPA1, vhaPPA2, vhaM9.7.1, and vhaM9.7.2are described. The Drosophila V-ATPase model is thus well-suited toboth forward and reverse genetic analysis of this complex multifunctionalenzyme.
REFERENCES
Anraku, Y., Hirata, R., Wada, Y., and Ohya, Y. (1992). J. Exp. Biol. 172, 67–81.
Ashburner, M., and Drysdale, R. (1994). Development 120, 2077–2079.
Ballinger, D. G., and Benzer, S. (1989). Proc. Natl. Acad. Sci. USA 86, 9402–9406.
Baron, R. (1995). Acta Orthopaed. Scand. 66, 66–70.
Bellen, H. J., and Smith, R. F. (1995). Trends Genet 11, 456–457.
Bowman, E. J., Tenney, K., and Bowman, B. J. (1988). J. Biol. Chem. 263, 13994–14001.
Brand, A. H., and Perrimon, N. (1993). Development 118, 401–415.
Brown, D., and Breton, S. (1996). J. Exp. Biol. 199, 2345–2358.
Brown, D., Sabolic, I., and Gluck, S. (1992). J. Exp. Biol. 172, 231–243.
Cooley, L., Kelley, R., and Spradling, A. (1988). Science 239, 1121–1128.
Davies, S. A., Huesmann, G. R., Maddrell, S. H. P., O'Donnell, M. J., Skaer, N. J. V., Dow, J. A. T., and Tublitz, N. J. (1995). Am. J. Physiol. 269, R1321–R1326.
Davies, S. A., Kelly, D. C., Goodwin, S. F., Wang, S.-Z., Kaiser, K., and Dow, J.A. T. (1996). J. Biol. Chem. 271, 30677–30684.
Davies, S. A., Stewart, E. J., Heusmann, G. R., Skaer, N. J. V., Maddrell, S. H. P., Tublitz, N. J., and Dow, J. A. T. (1997). Am. J. Physiol. 273, R823–R827.
Dow, J. A. T. (1984). Am. J. Physiol. 246, R633–R635.
Dow, J. A. T. (1994). In Organellar Proton-ATPases (Nelson, N., ed.), R. G. Landes Company, Austin, TX, pp. 75–102.
Dow, J. A. T., and Harvey, W. R. (1988). J. Exp. Biol. 140, 455–463.
Dow, J. A. T., Maddrell, S. H. P., Davies, S.-A., Skaer, N. J. V., and Kaiser, K. (1994a). Am. J. Physiol. 266, R1716–R1719.
Dow, J. A. T., Maddrell, S. H. P., Görtz, A., Skaer, N. V., Brogan, S., and Kaiser, K. (1994b). J. Exp. Biol. 197, 421–428.
Dow, J. A. T., Davies, S. A., Guo, Y., Graham, S., Finbow, M. E., and Kaiser, K. (1997). J. Exp. Biol. 202, 237–245.
Dow, J. A. T., Davies, S. A., and Sözen, M. A. (1998). Am. Zool. 38, 450–460.
Ferea, T. L., and Bowman, B. J. (1996). Genetics 143, 147–154.
Finbow, M. E., and Harrison, M. A. (1997). Biochem. J. 324, 697–712.
Gausz, J., Bencze, G., Gyurkovics, H., Ashburner, M., Ish-Horowitz, D., and Holden, J. J. (1979). Genetics 93, 917–934.
Gluck, S. (1992). J. Exp. Biol. 172, 29–37.
Gluck, S. L., Underhill, D. M., Iyori, M., Holliday, L. S., Kostrominova, T. Y., and Lee, B. S. (1996). Annu. Rev. Physiol. 58, 427–445.
Gogarten, J. P., Fichmann, J., Braun, Y., Morgan, L., Styles, P., Taiz, S. L., DeLapp, K., and Taiz, L. (1992). Plant Cell 4, 851–864.
Guo, Y., Gillan, A., Töiök, T., Kiss, I., Dow, J. A. T., and Kaiser, K. (1996a). Genome Res. 6, 972–979.
Guo, Y., Kaiser, K., Wieczorek, H., and Dow, J. A. T. (1996b). Gene 172, 239–243.
Guo, Y., Wang, Z., Carter, A., Kaiser, K., and Dow, J. A. T. (1996c). Biochim. Biophys. Acta 1283, 4–9.
Harvey, W. R., and Wieczorek, H. (1997). J. Exp. Biol. 200, 203–216.
Harvey, W. R., Cioffi, M., and Wolfersberger, M. G. (1981). Am. Zool. 21, 775–791.
Harvie, P. D., and Bryant, P. J. (1996). Conf. Drosophila Res. 37, 92.
Hirata, R., Graham, L. A., Takatsuki, A., Stevens, T. H., and Anraku, Y. (1997). J. Biol. Chem. 272, 4795–4803.
Kaiser, K., and Goodwin, S. F. (1990). Proc. Natl. Acad. Sci. USA 87, 1686–1690.
Kane, P. M. (1992). J. Exp. Biol. 172, 93–103.
Kato-Yamada, Y., Noji, H., Yasuda, R., Kinosita, K., Jr., and Yoshida, M. (1998). J Biol. Chem. 273, 19375–19377.
Klein, U. (1992). J. Exp. Biol. 172, 345–354.
Low, R., Rockel, B., Kirsch, M., Ratajczak, R., Hortensteiner, S., Martinoia, E., Luttge, U., and Rausch, T. (1996). Plant Physiol. 110, 259–265.
Meagher, L., McLean, P., and Finbow, M. E. (1990). Nucleic Acids Res. 18, 6712.
Mellman, I. (1992). J. Exp. Biol. 172, 39–45.
Nelson, N., and Taiz, L. (1989). Trends Biochem. Sci. 14, 113–116.
Noji, H., Yasuda, R., Yoshida, M., and Kinosita, K., Jr. (1997). Nature 386, 299–302.
Noumi, T., Beltran, C., Nelson, H., and Nelson, N. (1991). Proc. Natl. Acad. Sci. USA 88, 1938–1942.
O'Donnell, M. J., Dow, J. A. T., Huesmann, G. R., Tublitz, N. J., and Maddrell, S. H. P. (1996). J. Exp. Biol. 199, 1163–1175.
O'Donnell, M. J., Rheault, M. R., Davies, S. A., Rosay, P., Harvey, B. J., Maddrell, S. H. P., Kaiser, K., and Dow, J. A. T. (1998). Am. J. Physiol. 43, R1039–R1049.
Petrov, D. A., and Hartl, D. L. (1997). Gene 205, 279–289.
Rubin, G. M. (1988). Science 240, 1453–1459.
Rubin, G. M. (1996). Genome Res 6, 71–79.
Rubin, G. M. (1998). Trends Genet. 14, 340–343.
Rubin, G. M., and Spradling, A. C. (1982). Science 218, 348–353.
Ryerse, J. S. (1978). Nature 271, 745–746.
Sözen, M. A., Armstrong, J. D., Yang, M. Y., Kaiser, K., and Dow, J. A. T. (1997). Proc. Natl. Acad. Sci. USA 94, 5207–5212.
Spradling, A. C., Stern, D. M., Kiss, I., Roote, J., Laverty, T., and Rubin, G. M. (1995). Proc. Natl. Acad. Sci. USA 92, 10824–10830.
Sumner, J. P., Dow, J. A. T., Earley, F., Klein, U., Jäger, D., and Wieczorek, H. (1995). J. Biol. Chem. 270, 5649–5653.
Sze, H., Ward, J. M., Lai, S., and Perera, I. (1992). J. Exp. Biol. 172, 123–135.
Wieczorek, H., and Harvey, W. R. (1995). Physiol. Zool. 68, 15–23.
Wieczorek, H., Putzenlechner, M., Zeiske, W., and Klein, U. (1991). J. Biol. Chem. 266, 15340–15347.
Yasuda, R., Noji, H., Kinosita, K., Jr., Motojima, F., and Yoshida, M. (1997). Bioenerg. Biomembr. 29, 207–209.
Yasuda, R., Noji, H., Kinosita, K., Jr., and Yoshida, M. (1998). Cell 93, 1117–1124.
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Dow, J.A.T. The Multifunctional Drosophila melanogaster V-ATPase Is Encoded by a Multigene Family. J Bioenerg Biomembr 31, 75–84 (1999). https://doi.org/10.1023/A:1005400731289
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DOI: https://doi.org/10.1023/A:1005400731289