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Overexpression of archaeal proteins in Escherichia coli

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Abstract

Six archaeal proteins containing a high number of Escherichia coli rare codons in their genes were not well expressed in E. coli. These genes showed a five to twenty-fold increase in production when expressed in the presence of a plasmid harboring and expressing the argU and ileX genes encoding rare tRNAs (tRNA arg(de)AGA/AGG and tRNA ile(de)AUA. © Rapid Science Ltd. 1998

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Authors and Affiliations

  1. Structural Biology Department, Physical Biosciences Division of Lawrence Berkeley National Laboratory and Department of Chemistry, University of California Berkeley, USA

    Rosalind Kim, Stanley Goldman, Hisao Yokota & Sung-Hou Kim

  2. Molecular and Cell Biology, University of California Berkeley, USA

    Steve J. Sandler & Alvin J. Clark

Authors
  1. Rosalind Kim
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  2. Steve J. Sandler
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  3. Stanley Goldman
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  4. Hisao Yokota
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  5. Alvin J. Clark
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  6. Sung-Hou Kim
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Kim, R., Sandler, S.J., Goldman, S. et al. Overexpression of archaeal proteins in Escherichia coli. Biotechnology Letters 20, 207–210 (1998). https://doi.org/10.1023/A:1005305330517

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  • DOI: https://doi.org/10.1023/A:1005305330517

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