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Shifts in postembryonic somatic ploidy levels in Daphnia pulex

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Abstract

Although the presence of polyploid cells in somatic tissues of the cladoceran Daphnia has been reported, the nature of ploidy level shifts through life has not been investigated. This study examined the effects of growth and age on ploidy level variation across 10 instars in Daphnia pulex. The transition from mitotic to endomitotic cycles occurred during embryogenesis in all tissues, but the timing of it appeared to be tissue-specific. Lower than expected DNA estimates for the 4C and 8C ploidy classes suggested an underreplication of the genome during the first two endomitotic cycles, while complete replication seemed to occur in subsequent cycles. Polyploid cells underwent from four to seven endomitotic cycles over the 13 postembryonic instars, with ploidy levels reaching a maximum of 2048C in the labrum. The number of polyploid cells in all tissues, except the digestive tract, remained uniform beyond the fourth instar. Three patterns of ploidy level increments were observed i n the various tissues. Polyploid cells in the epipodite and rostrum exhibited age-related shifts, reaching asymptotic levels, while those in the digestive tract similarly reached asymptotic levels but then re-entered the mitotic cycle to enable tissue growth. Finally, ploidy level shifts in both the labrum and the epidermis were affected by age and food levels.

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References

  • Avancini, R. M. P. & M. L. S. Mello, 1990. Endopolyploidy in follicle epithelial cells around the nurse chamber during oogenesis in Chrysomya putoria (Diptera, Calliphoridae). Genetica 81: 5–10.

    Article  PubMed  CAS  Google Scholar 

  • Beaton, M. J. & P. D. N. Hebert, 1988. Geographic parthenogenesis and polyploidy in Daphnia pulex. Am. Nat. 132: 837–845.

    Article  Google Scholar 

  • Beaton, M. J. & P. D. N. Hebert, 1989. Miniature genomes and endopolyploidy in cladoceran crustaceans. Genome 32: 1048–1053.

    Google Scholar 

  • Beaton, M. J. & P. D. N. Hebert, 1994a. Patterns of DNA synthesis and mitotic activity during the intermoult of Daphnia. J. exp. Zool. 268: 400–409.

    Article  PubMed  CAS  Google Scholar 

  • Beaton, M. J. & P. D. N. Hebert, 1994b. Variation in chromosome numbers of Daphnia. Hereditas 120: 275–279.

    Article  Google Scholar 

  • Bradley, I. C., N. Perrin & P. Calow, 1991. Energy allocation in the cladoceran Daphnia magna Straus, under starvation and refeeding. Oecologia 86: 414–418.

    Article  Google Scholar 

  • Brodsky, V. Ya. & I. V. Uryvaeva, 1985. Genome Multiplication in Growth and Development. Biology of Polyploid and Polytene Cells. Development and Cell Biology Series; no. 15. Cambridge University Press, Cambridge.

    Google Scholar 

  • Carlson, J. & R. Abraham, 1985. Nuclear ploidy of neonatal rat livers: effects of two hepatic carcinogens (mirex and dimethynitrosamine). J. Tox. Env. Health 15: 551–559.

    Article  CAS  Google Scholar 

  • Coggeshall, R. E., B. A. Yaksta & F. J. Swartz, 1970. A cytophotometric analysis of the DNA in the nucleus of the giant cell, R-2, in Aplysia. Chromosoma (Berl.) 32: 205–212.

    CAS  Google Scholar 

  • De Rocher, E. J., K. R. Harkins, D. W. Galbraith & H. J. Bohnert, 1990. Developmentally regulated systemic endopolyploidy in succulents with small genomes. Science 250: 99–101.

    CAS  PubMed  Google Scholar 

  • Dittmann, F., P. H. Kogan & H. M. Hagedorn, 1989. Ploidy levels and DNA synthesis in fat body cells of the adult mosquito, Aedes aegypti: the role of juvenile hormone. Arch. Insect Biochem. Physiol. 12: 133–143.

    Article  CAS  Google Scholar 

  • Dittman, F. & E. Maier, 1987. Developmental patterns of DNAaccumulation and nuclear division in the follicle epithelium of the red cotton bug, Dysdercus intermedius (Heteroptera). J. Insect Physiol. 33: 191–200.

    Article  Google Scholar 

  • Ebert, D., 1992. A food-independent maturation threshold and size at maturity in Daphnia magna. Limnol. Oceanogr. 37: 878–881.

    Article  Google Scholar 

  • Fryer, G., 1962. Secretions of the labral and trunk limb glands in the cladoceran Eurycercus lamellatus. Nature, Lond. 195: 97.

    Article  Google Scholar 

  • Fryer, G., 1991. Functional morphology and the adaptive radiation of the Daphniidae (Branchiopoda: Anomopoda). Phil. Trans. r. Soc., Lond. B 331: 1–99.

    Google Scholar 

  • Galbraith, D. W., K. R. Harkins & S. Knapp, 1991. Systemic endopolyploidy in Arabidopsis thaliana. Pl. Phys. 96: 985–989.

    Article  CAS  Google Scholar 

  • Hebert, P. D. N. & T. J. Crease, 1980. Clonal coexistence in Daphnia pulex (Leydig): another planktonic paradox. Science 207: 1363–1365.

    Google Scholar 

  • Howarth, K. L. & L. M. Riddiford, 1991. Cellular differentiation of specialized epidermal cells: the dermal glands. In K. Binnington & A. Retnakaran (eds), The Physiology of the Insect Epidermis, CSIRO, Australia.

    Google Scholar 

  • Jäger, G., 1934. Ñber den Fettkörper von Daphnia magna. Z. Zellforsch. 22: 89–131.

    Article  Google Scholar 

  • Kiknadze, I. I., I. E. Vlasova & A. I. Sherudilo, 1975. Quantitative analysis of DNA content in the salivary gland chromosomes of Chironomus thummi at larval and prepupal stages. Cell Different. 3: 323–334.

    Article  CAS  Google Scholar 

  • Kooman, C. & K. K. Nair, 1982. Polyploidization in the fat body of the desert locust Schistocerca gregaria during growth and metamorphosis. Cytobios 34: 25–34.

    Google Scholar 

  • Kowles, R. V., F. Srienc & R. L. Phillips, 1990. Endoreduplication of nuclear DNA in the developing maize endosperm. Develop. Gen. 11: 125–132.

    Article  CAS  Google Scholar 

  • Lampert, W., 1993. Phenotypic plasticity of the size at first reproduction in Daphnia: the importance of maternal size. Ecology 74: 1455–1466.

    Article  Google Scholar 

  • LaPointe, M. C., J. K. Koeppe & K. K. Nair, 1985. Follicle cell polyploidy in Leucophaea maderae: regulation by juvenile hormone. J. Insect. Physiol. 31: 187–193.

    Article  CAS  Google Scholar 

  • Matlock, D. B. & E. J. Dornfeld, 1982. The effect of crustecdysone on DNA synthesis in polyploid somatic cells of an isopod. Comp. Biochem. Physiol. 73B: 603–605.

    CAS  Google Scholar 

  • Meyer, R. & W. Nagl, 1993. Somatic polyploidy in extraembryonic membranes of the snake, Elaphe guttata. Protoplasma 172: 132–135.

    Article  Google Scholar 

  • Nagl, W., 1978. Endopolyploidy and polyteny in differentiation and evolution. North-Holland Publ., Amsterdam.

    Google Scholar 

  • Roberts, B., J. M. Whitten & L. I. Gilbert, 1974. DNA synthesis patterns in the giant foot-pad nuclei of Sarcophaga bullata (Sarcophagidae, Diptera). Chromosoma (Berl.) 47: 193–210.

    Article  CAS  Google Scholar 

  • Sin, W. C. & J. Pasternak, 1970. Number and DNA content of nuclei in the free-living nematode Panagrellus silusiae at each stage during postembryonic development. Chromosoma (Berl.) 32: 191–204.

    CAS  Google Scholar 

  • Smith, A. V. & T. L. Orr-Weaver, 1991. The regulation of the cell cycle during Drosophila embryogenesis: the transition to polyteny. Development 112: 997–1008.

    PubMed  CAS  Google Scholar 

  • Stevenson, J. R., 1985. Dynamics of the integument. In D. E. Bliss (ed.), The Biology of the Crustacea, vol. 9, Academic Press.

  • Van Oostveldt, P. & R. Van Parjis, 1975. The effect of light on nucleic-acid synthesis and polyploidy level in elongating epicotyl cells of Pisum sativum. Planta (Berl.) 124: 287–295.

    CAS  Google Scholar 

  • Welch, R. M., 1957. A developmental analysis of the lethal mutant l(2)gl of Drosophila melanogaster based on cytophotometric determination of nuclear desoxyribonucleic acid (DNA) content. Genetics 42: 544–559.

    CAS  PubMed  Google Scholar 

  • Zaffagnini, F., 1987. Reproduction in Daphnia. Mem. Ist. Ital. Idrobiol. 45: 245–284.

    Google Scholar 

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Beaton, M.J., Hebert, P.D.N. Shifts in postembryonic somatic ploidy levels in Daphnia pulex. Hydrobiologia 394, 29–39 (1999). https://doi.org/10.1023/A:1003567012950

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