Skip to main content
SpringerLink
Log in

Elongated conformers of charge states +11 to +15 of bovine ubiquitin studied using ESI-FAIMS-MS

  • Published:
Journal of the American Society for Mass Spectrometry

Abstract

Recent advancements in high-field asymmetric waveform ion mobility spectrometry (FAIMS) have led to significant improvements in the application of this technology to the study of protein conformers. Compared with previous work, the maximum value of the separation voltage (i.e., the dispersion voltage) has increased, thereby enabling multiple, elongated conformers of individual charge states of bovine ubiquitin to be separated in the gas phase (e.g., four conformers of each of the +11 and +12 charge states were separated). The use of a carrier gas mixture of 40% nitrogen and 60% helium changed the separation selectivity compared with pure nitrogen and enhanced the signal intensity, especially for the +14 and +15 charge states (the latter was not detected in a nitrogen carrier gas). Conformer cross sections were determined using the FAIMS/energy-loss method and found to be similar within a given charge state. The cross sections for conformers of charge states +13, +14, and +15 plateau at about 2000 Å2 suggesting that the structure of bovine ubiquitin is essentially unfolded after the addition of the 13th proton.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. In Ubiquitin; Rechsteiner, M., Ed., Plenum Press: New York, 1988; pp. 1–3.

    Google Scholar 

  2. Goldberg, A. L. Science 1995, 268, 522–523.

    Article  CAS  Google Scholar 

  3. Hochstrasser, M. Curr. Opin. Cell Biol. 1995, 7, 215–223.

    Article  CAS  Google Scholar 

  4. Argiles, J. M.; Lopez-Soriano, F. J. Trends Pharmacol. Sci. 1996, 17, 223–226.

    Article  CAS  Google Scholar 

  5. Hasselgren, P. O.; Fischer, J. E. Ann. Surg. 1997, 225, 307–316.

    Article  CAS  Google Scholar 

  6. Wilkinson, K. D. J. Nutr. 1999, 129, 1933–1936.

    CAS  Google Scholar 

  7. Attaix, D.; Combaret, L.; Tilignac, T.; Taillandier, D. Mol. Biol. Rep. 1999, 26, 77–82.

    Article  CAS  Google Scholar 

  8. Gieniec, J.; Mack, L. L.; Nakamae, K.; Gupta, C.; Kumar, V.; Dole, M. Biomed. Mass Spectrom. 1984, 11, 259–268.

    Article  CAS  Google Scholar 

  9. Fenn, J. B.; Mann, M.; Meng, C. K.; Wong, S. F.; Whitehouse, C. M. Science 1989, 246, 64–71.

    Article  CAS  Google Scholar 

  10. Karas, M.; Bachman, U.; Hillenkamp, F. Int. J. Mass Spectrom. Ion Proc. 1987, 78, 53–81.

    Article  CAS  Google Scholar 

  11. Wolynes, P. G. Proc. Natl. Acad. Sci. USA 1995, 92, 2426–2427.

    Article  CAS  Google Scholar 

  12. Valentine, S. J.; Counterman, A. E.; Clemmer, D. E. J. Am. Soc. Mass Spectrom. 1997, 8, 954–961.

    Article  CAS  Google Scholar 

  13. Li, J. W.; Taraszka, J. A.; Counterman, A. E.; Clemmer, D. E. Int. J. Mass Spectrom. 1999, 187, 37–47.

    Article  Google Scholar 

  14. Suckau, D.; Shi, Y.; Beu, S. C.; Senko, M. W.; Quinn, J. P.; Wampler III, F. M.; McLafferty, F. W. Proc. Natl. Acad. Sci. USA 1993, 90, 790–793.

    Article  CAS  Google Scholar 

  15. Freitas, M. A.; Hendrickson, C. L.; Emmett, M. R.; Marshall, A. G. Int. J. Mass Spectrom. 1999, 187, 565–575.

    Article  Google Scholar 

  16. Cassady, C. J.; Carr, S. R. J. Mass Spectrom. 1996, 31, 247–254.

    Article  CAS  Google Scholar 

  17. Clemmer, D. E.; Jarrold, M. F. J. Mass Spectrom. 1997, 32, 577–592.

    Article  CAS  Google Scholar 

  18. Winger, B. E.; Light-Wahl, K. J.; Rockwood, A. L.; Smith, R. D. J. Am. Chem. Soc. 1992, 114, 5897–5898.

    Article  CAS  Google Scholar 

  19. Green, M. K.; Lebrilla, C. B. Mass Spectrom. Reviews 1997, 16, 53–71.

    Article  CAS  Google Scholar 

  20. Hoaglund-Hyzer, C. S.; Counterman, A. E.; Clemmer, D. E. Chem. Rev. 1999, 99, 3037–3079.

    Article  CAS  Google Scholar 

  21. Purves, R. W.; Guevremont, R.; Barnett, D. Int. J. Mass Spectrom. 2000, 197, 163–177.

    Article  CAS  Google Scholar 

  22. Purves, R. W.; Barnett, D. A.; Ells, B.; Guevremont, R. J. Am. Soc. Mass Spectrom. 2000, 11, 738–745.

    Article  CAS  Google Scholar 

  23. Buryakov, I. A.; Krylov, E. V.; Nazarov, E. G.; Rasulev, U. K. Int. J. Mass Spectrom. Ion Proc. 1993, 128, 143–148.

    Article  CAS  Google Scholar 

  24. Purves, R. W.; Guevremont, R.; Day, S.; Pipich, C. W.; Matyjaszczyk, M. S. Rev. Sci. Instrum. 1998, 69, 4094–4105.

    Article  CAS  Google Scholar 

  25. Purves, R. W.; Guevremont, R. Anal. Chem. 1999, 71, 2346–2357.

    Article  CAS  Google Scholar 

  26. Covey, T.; Douglas, D. J. J. Am. Soc. Mass Spectrom. 1993, 4, 616–623.

    Article  CAS  Google Scholar 

  27. Chen, Y.; Collings, B. A.; Douglas, D. J. J. Am. Soc. Mass Spectrom. 1997, 8, 681–687.

    Article  CAS  Google Scholar 

  28. Barnett, D. A.; Purves, R. W.; Ells, B.; Guevremont, R. J. Mass Spectrom. 2000, 35, 976–980.

    Article  CAS  Google Scholar 

  29. Barnett, D. A.; Ells, B.; Guevremont, R.; Purves, R. W.; Viehland, L. A. J. Am. Soc. Mass Spectrom. 2000, 11, 1125–1133.

    Article  CAS  Google Scholar 

  30. Ells, B.; Barnett, D. A.; Purves, R. W.; Guevremont, R. Anal. Chem. 2000, 72, 4555–4559.

    Article  CAS  Google Scholar 

  31. McCooeye, M. A.; Ells, B.; Barnett, D. A.; Purves, R. W.; Guevremont, R. J. Anal. Toxicol. 2000, 25, 81–87.

    Google Scholar 

  32. Stalder, J. R., Zurick, V. J. National Advisory Committee for Aeronautics TN 2423, NACA, 1951.

  33. Guevremont, R.; Purves, R. W. Rev. Sci. Instrum. 1999, 70, 1370–1383.

    Article  CAS  Google Scholar 

  34. Shelimov, K. B.; Clemmer, D. E.; Hudgins, R. R.; Jarrold, M. F. J. Am. Chem. Soc 1997, 119, 2240–2248.

    Article  CAS  Google Scholar 

  35. Viehland, L. A.; Mason, E. A. Ann. Phys. 1975, 91, 499–533.

    Article  CAS  Google Scholar 

  36. Viehland, L. A.; Mason, E. A. Ann. Phys. 1978, 110, 287–328.

    Article  CAS  Google Scholar 

  37. Guevremont, R.; Siu, K. W. M.; Wang, J.; Ding, L. Anal. Chem. 1997, 69, 3959–3965.

    Article  CAS  Google Scholar 

  38. Harrison, A. G. Mass Spectrom. Rev. 1997, 16, 201–217.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute for National Measurement Standards, National Research Council of Canada, K1A 0R6, Ottawa, ON, Canada

    Randy W. Purves, David A. Barnett, Barbara Ells & Roger Guevremont

Authors
  1. Randy W. Purves
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. David A. Barnett
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Barbara Ells
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Roger Guevremont
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Roger Guevremont.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Purves, R.W., Barnett, D.A., Ells, B. et al. Elongated conformers of charge states +11 to +15 of bovine ubiquitin studied using ESI-FAIMS-MS. J Am Soc Spectrom 12, 894–901 (2001). https://doi.org/10.1016/S1044-0305(01)00272-0

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1016/S1044-0305(01)00272-0

Keywords

Search

Navigation