Journal of the American Society for Mass Spectrometry

, Volume 21, Issue 2, pp 220–231

MALDI imaging of neutral cuticular lipids in insects and plants

Article

DOI: 10.1016/j.jasms.2009.10.003

Cite this article as:
Vrkoslav, V., Muck, A., Cvačka, J. et al. J Am Soc Mass Spectrom (2010) 21: 220. doi:10.1016/j.jasms.2009.10.003

Abstract

The spatial distribution of neutral lipids and hydrocarbons has been imaged using MALDI-TOF mass spectrometry on intact plant and insect surfaces, namely wings and legs of the gray flesh fly (Neobellieria bullata), wings of common fruit fly (Drosophila melanogaster), leaves of thale cress (Arabidopsis thaliana), and leaves of date palm tree (Phoenix sp.). The distribution of wax esters (WEs) and saturated and unsaturated hydrocarbons (HCs) was visualized. The samples were attached on a target and multiply sprayed with lithium or sodium 2,5-dihydroxybenzoate. The deposits were homogenous, consisting of small islands (50–150 µm) of matrix crystals separated by small areas (10 µm) of uncovered cuticle. Samples of N. bullata wings were found to contain HCs and WEs distributed close to their basal parts. The distribution of sodium and potassium ions was visualized on samples prepared by sublimation of 2,5-dihydroxybenzoic acid. Pheromonal dienes were detected on D. melanogaster female wings. A homogenous distribution of saturated WEs was observed on A. thaliana and Phoenix sp. leaf samples. The optimum number of laser shots per pixel was found to be higher than for polar compounds imaging.

Download to read the full article text

Supplementary material

13361_2011_210200220_MOESM1_ESM.doc (1.2 mb)
Supplementary material, approximately 1230 KB.

Copyright information

© American Society for Mass Spectrometry 2010

Authors and Affiliations

  1. 1.Institute of Organic Chemistry and BiochemistryAcademy of Sciences of the Czech RepublicPragueCzech Republic
  2. 2.Max Planck Institute for Chemical EcologyMass Spectrometry GroupJenaGermany