Metabolite imaging using matrix-enhanced surface-assisted laser desorption/ionization mass spectrometry (ME-SALDI-MS)

  • Qiang Liu
  • Yongsheng Xiao
  • Coral Pagan-Miranda
  • Yu Matthew Chiu
  • Lin He
Articles

DOI: 10.1016/j.jasms.2008.09.011

Cite this article as:
Liu, Q., Xiao, Y., Pagan-Miranda, C. et al. J Am Soc Mass Spectrom (2009) 20: 80. doi:10.1016/j.jasms.2008.09.011

Abstract

We describe here the use of a hybrid ionization approach, matrix-enhanced surface-assisted laser desorption/ionization mass spectrometry (ME-SALDI-MS) in bioimaging. ME-SALDI combines the strengths of traditional matrix-assisted laser desorption/ionization (MALDI) and SALDI and enables successful MS imaging of low-mass species with improved detection sensitivity. Using 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) as the MS standard, MS performances of MALDI, SALDI, and ME-SALDI are systematically compared. The analyte desorption and ionization mechanism in ME-SALDI is qualitatively speculated based on the observation of significantly reduced matrix background and improved survival yields of molecular ions. Improvements in detection sensitivity of low-mass species using ME-SALDI over MALDI in imaging are demonstrated with mouse heart and brain tissues.

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Supplementary material

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Copyright information

© American Society for Mass Spectrometry 2009

Authors and Affiliations

  • Qiang Liu
    • 1
  • Yongsheng Xiao
    • 1
  • Coral Pagan-Miranda
    • 1
  • Yu Matthew Chiu
    • 1
  • Lin He
    • 1
  1. 1.Department of ChemistryNorth Carolina State UniversityRaleigh
  2. 2.Department of ChemistryUniversity of Puerto RicoSan Juan
  3. 3.Lab Corporation of AmericaDurham