Journal of the American Society for Mass Spectrometry

, Volume 20, Issue 1, pp 112–123

Detection of honeybee venom in envenomed tissues by direct MALDI MSI

Authors

    • Interdepartmental Centre of Mass SpectrometryUniversity of Florence
  • Duccio Lambardi
    • Department of Evolutionary BiologyUniversity of Florence
  • Guido Mastrobuoni
    • Interdepartmental Centre of Mass SpectrometryUniversity of Florence
  • Giancarlo la Marca
    • Department of PharmacologyUniversity of Florence, Mass Spectrometry Laboratory, Metabolic and Neuromuscular Unit, Meyer Children’s Hospital
  • Gloriano Moneti
    • Interdepartmental Centre of Mass SpectrometryUniversity of Florence
  • Stefano Turillazzi
    • Department of Evolutionary BiologyUniversity of Florence
Articles

DOI: 10.1016/j.jasms.2008.09.006

Cite this article as:
Francese, S., Lambardi, D., Mastrobuoni, G. et al. J Am Soc Mass Spectrom (2009) 20: 112. doi:10.1016/j.jasms.2008.09.006

Abstract

A new analytical approach using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) for the study of honeybee venom is shown. In vitro and in vivo models simulating the bee sting have been developed using live honeybees and, as the envenomation sites, pig ears and rat legs; MALDI MSI has been used to map, over time, the diffusion and distribution of three venom allergens (Api m 1, Api m 4, and Api m 6) and two venom toxins (apamine and mast cell degranulating peptide). In conjunction with other classical biochemical techniques and high resolution mass spectrometry (HRMS), structural data have been obtained that contribute to current understanding of honeybee venom composition. Initial data have also been obtained demonstrating the feasibility of mapping the organism’s response to the sting. The opportunity to monitor venom diffusion and the organism’s response at the same time might open new pathways for in vivo preclinical studies in designing and testing new venom immunotherapy (VIT).

Supplementary material

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Supplementary material, approximately 126 KB.

Copyright information

© American Society for Mass Spectrometry 2009