A linear ion trap mass spectrometer with versatile control and data acquisition for ion/ion reactions
- Cite this article as:
- Soyk, M.W., Zhao, Q., Houk, R.S. et al. J Am Soc Mass Spectrom (2008) 19: 1821. doi:10.1016/j.jasms.2008.08.020
A linear ion trap (LIT) with electrospray ionization (ESI) for top-down protein analysis has been constructed. An independent atmospheric sampling glow discharge ionization (ASGDI) source produces reagent ions for ion/ion reactions. The device is also meant to enable a wide variety of ion/ion reaction studies. To reduce the instrument’s complexity and make it available for wide dissemination, only a few simple electronics components were custom built. The instrument functions as both a reaction vessel for gas-phase ion/ion reactions and a mass spectrometer using mass-selective axial ejection. Initial results demonstrate trapping efficiency of 70% to 90% and the ability to perform proton transfer reactions on intact protein ions, including dual polarity storage reactions, transmission mode reactions, and ion parking.