A study of noncovalent protein complexes by matrix-assisted laser desorption/ionization
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- Song, F. J Am Soc Mass Spectrom (2007) 18: 1286. doi:10.1016/j.jasms.2007.03.026
A sample preparation method has been developed for detection of noncovalent protein—protein complexes by MALDI in this work. An aqueous solution of the matrix at pH 7 allows the reproducible detection of a protein dimer, a protein tetramer, and a heterodimer. The signals are stable under long irradiation and can be detected at wide ranges of concentrations and with different laser intensities.