Journal of the American Society for Mass Spectrometry

, Volume 17, Issue 1, pp 96–103

Structural analysis of κ-carrageenan sulfated oligosaccharides by positive mode Nano-ESI-FTICR-MS and MS/MS by SORI-CID

  • Jennifer T. Aguilan
  • Fabian M. Dayrit
  • Jinhua Zhang
  • Milady R. Niñonuevo
  • Carlito B. Lebrilla
Articles

DOI: 10.1016/j.jasms.2005.09.009

Cite this article as:
Aguilan, J.T., Dayrit, F.M., Zhang, J. et al. J Am Soc Mass Spectrom (2006) 17: 96. doi:10.1016/j.jasms.2005.09.009

Abstract

Structural analysis of sulfated oligosaccharides from κ-carrageenan of up to ten residues (MW >2 kDa) was successfully carried out by positive mode nano-ESI-FTICR-MS together with MS/MS using sustained off-resonance irradiation-collision induced dissociation (SORI-CID). Glycosidic bond cleavage reactions via the B- and Y-types of fragmentation were observed and enabled complete sequencing of the oligosaccharide samples. The positions of the labile sulfate substituents were observable using SORI-CID, enabling the determination of the sequence of the sulfated residues.

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© American Society for Mass Spectrometry 2005

Authors and Affiliations

  • Jennifer T. Aguilan
    • 1
  • Fabian M. Dayrit
    • 1
  • Jinhua Zhang
    • 2
  • Milady R. Niñonuevo
    • 2
  • Carlito B. Lebrilla
    • 2
  1. 1.Department of ChemistryAteneo de Manila UniversityQuezon CityPhilippines
  2. 2.Department of ChemistryUniversity of California at DavisDavisUSA