Using oxidized carbon nanotubes as matrix for analysis of small molecules by MALDI-TOF MS

  • Chensong Pan
  • Songyun Xu
  • Ligang Hu
  • Xingye Su
  • Junjie Ou
  • Hanfa Zou
  • Zhong Guo
  • Yu Zhang
  • Baochuan Guo
Articles

DOI: 10.1016/j.jasms.2005.03.009

Cite this article as:
Pan, C., Xu, S., Hu, L. et al. J Am Soc Mass Spectrom (2005) 16: 883. doi:10.1016/j.jasms.2005.03.009

Abstract

Oxidized carbon nanotubes are tested as a matrix for analysis of small molecules by matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). Compared with nonoxidized carbon nanotubes, oxidized carbon nanotubes facilitate sample preparation because of their higher solubility in water. The matrix layer of oxidized carbon nanotubes is much more homogeneous and compact than that of nonoxidized carbon nanotubes. The efficiency of desorption/ionization for analytes and the reproducibility of peak intensities within and between sample spots are greatly enhanced on the surface of oxidized carbon nanotubes. The advantage of the oxidized carbon nanotubes in comparison with α-cyano-4-hydroxycinnamic acid (CCA) and carbon nanotubes is demonstrated by MALDI-TOF-MS analysis of an amino acid mixture. The matrix is successfully used for analysis of synthetic hydroxypropyl β-cyclodextrin, suggesting a great potential for monitoring reactions and for product quality control. Reliable quantitative analysis of jatrorrhizine and palmatine with a wide linear range (1–100 ng/mL) and good reproducibility of relative peak areas (RSD less than 10%) is achieved using this matrix. Concentrations of jatrorrhizine (8.65 mg/mL) and palmatine (10.4 mg/mL) in an extract of Coptis chinensis Franch are determined simultaneously using the matrix and a standard addition method.

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Copyright information

© American Society for Mass Spectrometry 2005

Authors and Affiliations

  • Chensong Pan
    • 1
  • Songyun Xu
    • 1
  • Ligang Hu
    • 1
    • 2
  • Xingye Su
    • 1
  • Junjie Ou
    • 1
  • Hanfa Zou
    • 1
  • Zhong Guo
    • 1
  • Yu Zhang
    • 1
  • Baochuan Guo
    • 1
    • 3
  1. 1.National Chromatographic Research and Analysis Center, Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalianChina
  2. 2.Research Center for Eco-Environmental ScienceChinese Academy of SciencesBeijingChina
  3. 3.Department of ChemistryCleveland State UniversityClevelandUSA

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