Account And Perspective

Journal of the American Society for Mass Spectrometry

, Volume 10, Issue 2, pp 91-103

Peptide and protein identification by matrix-assisted laser desorption ionization (MALDI) and MALDI-post-source decay time-of-flight mass spectrometry

  • P. ChaurandAffiliated withInstitute of Laser Medicine, University of Duesseldorf
  • , F. LuetzenkirchenAffiliated withInstitute of Laser Medicine, University of Duesseldorf
  • , B. SpenglerAffiliated withBiologisch-Medizinisches Forschungszentrum, University of Duesseldorf Email author 

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Abstract

The potential of matrix-assisted laser desorption ionization (MALDI) and MALDI-post-source decay (PSD) time-of-flight mass spectrometry for the characterization of peptides and proteins is discussed. Recent instrumental developments provide for levels of sensitivity and accuracy that make these techniques major analytical tools for proteome analysis. New software developments employing protein database searches have greatly enhanced the fields of application of MALDI-PSD. Peptides and proteins can be easily identified even if only a partial sequence information is determined. Derivatization procedures have been optimized for MALDI-PSD to increase the structural information and to obtain a complete peptide sequence even in critical cases. They are fast, simple and can be performed on target. MALDI-PSD is also a very powerful tool to characterize or elucidate post-translational or chemically induced modifications. In association with database searches, proteins issued from electrophoretic gels can be identified after specific enzymatic cleavages and peptide mapping.