Journal of the American Society for Mass Spectrometry

, Volume 13, Issue 9, pp 1015–1027

Practical quantitative biomedical applications of MALDI-TOF mass spectrometry

  • Martin Bucknall
  • Kim Y. C. Fung
  • Mark W. Duncan
Article

DOI: 10.1016/S1044-0305(02)00426-9

Cite this article as:
Bucknall, M., Fung, K.Y.C. & Duncan, M.W. J. Am. Soc. Spectrom. (2002) 13: 1015. doi:10.1016/S1044-0305(02)00426-9

Abstract

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOFMS) is used to obtain fast and accurate determinations of molecular mass, but quantitative determinations are generally made by other techniques. In this study we illustrate the practical utility of automated MALDI-TOFMS as a tool for quantifying a diverse array of biomolecules covering an extensive molecular weight range, and present in biological extracts and fluids. Growth hormone was measured in rat pituitary tissue; insulin in human pancreatic tissue; homovanillic acid in human urine; and LVV-hemorphin-7, epinephrine and norepinephrine in human adrenal and pheochromocytoma tissues. Internal standards including compounds of similar molecular weight, structural analogs or isotopomers were incorporated into each analysis. We report on the current practical limitations of quantitative MALDI-TOFMS and highlight some of the potential benefits of this technique as a quantitative tool.

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Copyright information

© American Society for Mass Spectrometry 2002

Authors and Affiliations

  • Martin Bucknall
    • 1
  • Kim Y. C. Fung
    • 1
  • Mark W. Duncan
    • 2
  1. 1.Ray Williams Biomedical Mass Spectrometry FacilityUniversity of New South WalesSydneyAustralia
  2. 2.Department of Pharmaceutical Sciences, School of Pharmacy, C238University of Colorado Health Sciences CenterDenverUSA