Abstract
A linear ion trap (LIT) with electrospray ionization (ESI) for top-down protein analysis has been constructed. An independent atmospheric sampling glow discharge ionization (ASGDI) source produces reagent ions for ion/ion reactions. The device is also meant to enable a wide variety of ion/ion reaction studies. To reduce the instrument’s complexity and make it available for wide dissemination, only a few simple electronics components were custom built. The instrument functions as both a reaction vessel for gas-phase ion/ion reactions and a mass spectrometer using mass-selective axial ejection. Initial results demonstrate trapping efficiency of 70% to 90% and the ability to perform proton transfer reactions on intact protein ions, including dual polarity storage reactions, transmission mode reactions, and ion parking.
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Douglas, D. J.; Frank, A. J.; Mao, D. M. Linear ion traps in mass spectrometry. Mass Spectrom. Rev. 2005, 24, 1–29.
Campbell, J. M.; Collings, B. A.; Douglas, D. J. A new linear ion trap time-of-flight system with tandem mass spectrometry capabilities. Rapid Commun. Mass Spectrom. 1998, 12, 1463–1474.
Collings, B. A.; Campbell, J. M.; Mao, D. M.; Douglas, D. J. A combined linear ion trap time-of-flight system with improved performance and MSn capabilities. Rapid Commun. Mass Spectrom. 2001, 15, 1777–1795.
Harkewicz, R.; Belov, M. E.; Anderson, G. A.; Pasa-Tolic, L.; Masselon, C. D.; Prior, D. C.; Udseth, H. R.; Smith, R. D. ESI-FTICR mass spectrometry employing data-dependent external ion selection and accumulation. J. Am. Soc. Mass Spectrom. 2002, 13, 144–154.
Syka, J. E. P.; Marto, J. A.; Bai, D. L.; Horning, S.; Senko, M. W.; Schwartz, J. C.; Ueberheide, B.; Garcia, B.; Busby, S.; Muratore, T.; Shabanowitz, J.; Hunt, D. F. Novel linear quadrupole ion trap/FT mass spectrometer: Performance characterization and use in the comparative analysis of histone H3 post-translational modifications. J. Proteome Res. 2004, 3, 621–626.
Schwartz, J. C.; Senko, M. W.; Syka, J. E. P. A two-dimensional quadrupole ion trap mass spectrometer. J. Am. Soc. Mass Spectrom. 2002, 13, 659–669.
Hager, J. W. A new linear ion trap mass spectrometer. Rapid Commun. Mass Spectrom. 2002, 16, 512–526.
Jonscher, K. R.; Yates, J. R. The quadrupole ion trap mass spectrometer—a small solution to a big challenge. Anal. Biochem. 1997, 244, 1–15.
Reid, G. E.; McLuckey, S. A. ‘Top down’ protein characterization via tandem mass spectrometry. J. Mass Spectrom. 2002, 37, 663–675.
Hu, Q. Z.; Noll, R. J.; Li, H. Y.; Makarov, A.; Hardman, M.; Cooks, R. G. The Orbitrap: a new mass spectrometer. J. Mass Spectrom. 2005, 40, 430–443.
Syka, J. E. P.; Coon, J. J.; Schroeder, M. J.; Shabanowitz, J.; Hunt, D. F. Peptide and protein sequence analysis by electron transfer dissociation mass spectrometry. Proc. Natl. Acad. Sci. U.S.A. 2004, 101, 9528–9533.
Xia, Y.; Wu, J.; McLuckey, S. A.; Londry, F. A.; Hager, J. W. Mutual storage mode ion/ion reactions in a hybrid linear ion trap. J. Am. Soc. Mass Spectrom. 2005, 16, 71–81.
Stephenson, J. L.; McLuckey, S. A. Simplification of product ion spectra derived from multiply charged parent ions via ion/ion chemistry. Anal. Chem. 1998, 70, 3533–3544.
Londry, F. A.; Hager, J. W. Mass selective axial ion ejection from a linear quadrupole ion trap. J. Am. Soc. Mass Spectrom. 2003, 14, 1130–1147.
Liang, X. R.; McLuckey, S. A. Transmission mode ion/ion proton transfer reactions in a linear ion trap. J. Am. Soc. Mass Spectrom. 2007, 18, 882–890.
Liang, X. R.; Han, H. L.; Xia, Y.; McLuckey, S. A. A pulsed triple ionization source for sequential ion/ion reactions in an electrodynamic ion trap. J. Am. Soc. Mass Spectrom. 2007, 18, 369–376.
Xia, Y.; Liang, X. R.; McLuckey, S. A. Pulsed dual electrospray ionization for ion/ion reactions. J. Am. Soc. Mass Spectrom. 2005, 16, 1750–1756.
Xia, Y.; Chrisman, P. A.; Erickson, D. E.; Liu, J.; Liang, X. R.; Londry, F. A.; Yang, M. J.; McLuckey, S. A. Implementation of ion/ion reactions in a quadrupole/time-of-flight tandem mass spectrometer. Anal. Chem. 2006, 78, 4146–4154.
Liang, X. R.; Hager, J. W.; McLuckey, S. A. Transmission mode ion/ion electron-transfer dissociation in a linear ion trap. Anal. Chem. 2007, 79, 3363–3370.
Liang, X. R.; Xia, Y.; McLuckey, S. A. Alternately pulsed nanoelectrospray ionization/atmospheric pressure chemical ionization for ion/ion reactions in an electrodynamic ion trap. Anal. Chem. 2006, 78, 3208–3212.
Han, H. L.; Xia, Y.; McLuckey, S. A. Ion trap collisional activation of c and z(center dot) ions formed via gas-phase ion/ion electron-transfer dissociation. J. Proteome. Res. 2007, 6, 3062–3069.
Wu, J.; Hager, J. W.; Xia, Y.; Londry, F. A.; McLuckey, S. A. Positive ion transmission mode ion/ion reactions in a hybrid linear ion trap. Anal. Chem. 2004, 76, 5006–5015.
Wells, J. M.; Chrisman, P. A.; McLuckey, S. A. “Dueling” ESI: Instrumentation to study ion/ion reactions of electrospray-generated cations and anions. J. Am. Soc. Mass Spectrom. 2002, 13, 614–622.
Badman, E. R.; Chrisman, P. A.; McLuckey, S. A. A quadrupole ion trap mass spectrometer with three independent ion sources for the study of gas-phase ion/ion reactions. Anal. Chem. 2002, 74, 6237–6243.
Zhao Q. Soyk M. W. Schieffer G. M. Houk R. S. Badman E. R. Fuhrer K. Gonin M. An ion trap-ion mobility-time of flight mass spectrometer with three ion sources for ion/ion reactions. J. Am. Soc. Mass Spectrom. 2008, unpublished.
Paul, W.; Reinhard, H. P.; Vonzahn, U. The electric mass filter as mass spectrometer and isotope separator. Zeitschrift fur Physik 1958, 152, 143–182.
McLuckey, S. A.; Reid, G. E.; Wells, J. M. Ion parking during ion/ion reactions in electrodynamic ion traps. Anal. Chem. 2002, 74, 336–346.
Chrisman, P. A.; Pitteri, S. J.; McLuckey, S. A. Parallel ion parking: Improving conversion of parents to first-generation products in electron transfer dissociation. Anal. Chem. 2005, 77, 3411–3414.
Chrisman, P. A.; Pitteri, S. J.; McLuckey, S. A. Parallel ion parking of protein mixtures. Anal. Chem. 2006, 78, 310–316.
Moradian, A.; Douglas, D. J. Mass selective axial ion ejection from linear quadrupoles with added octopole fields. J. Am. Soc. Mass Spectrom. 2008, 19, 270–280.
Stephenson, J. L.; McLuckey, S. A. Adaptation of the Paul Trap for study of the reaction of multiply charged cations with singly charged anions. Int. J. Mass Spectrom. Ion Processes 1997, 162, 89–106.
Mo, W.; Langford, M. L.; Todd, J. F. J. Investigation of ‘ghost’ peaks caused by nonlinear fields in the ion trap mass spectrometer. Rapid Commun. Mass Spectrom. 1995, 9, 107–113.
Franzen, J.; Gabling, R. H.; Schubert, M.; Wang, Y. In Practical Aspects of Ion Trap Mass Spectrometry, Vol. I; March, R. E.; Todd, J. F. J., Eds.; CRC Press: Boca Raton, FL, 1995; Vol. 1, p. 49–167.
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Published online September 6, 2008
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Soyk, M.W., Zhao, Q., Houk, R.S. et al. A linear ion trap mass spectrometer with versatile control and data acquisition for ion/ion reactions. J Am Soc Mass Spectrom 19, 1821–1831 (2008). https://doi.org/10.1016/j.jasms.2008.08.020
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DOI: https://doi.org/10.1016/j.jasms.2008.08.020