Abstract
The pattern of in vivo nitrate reductase (NR) activity in the individual leaf blades of Myrica esculenta was studied under 0.2 M phosphate buffer solutions with pH ranging from 6.8 to 8.4 and concentrations of substrate solutions KNO3 varying from 0.025, 0.050, 0.10, 0.20 and 0.25 M. Phosphate buffer 0.10 M solutions of pH ranging from 6.0 to 8.4 were also tested with the 0.10, 0.15 and 0.20 substrate (KNO3) concentrations. Highest NR activity was observed with 0.10 M buffer concentration, pH 8.0, and substrate (KNO3) concentration 0.10 M. Among different leaf blades from top to bottom, NR activity increased up to 8th leaf position, and afterwards it decreased. Fresh and dry weight was higher between 6th–9th leaves. However, moisture content remained between 30 and 40 % in all the leaves.
References
Abrol, Y. P., Kaim, M. S., & Nair, T. V. R. (1976). Nitrogen assimilation its mobilisation and accumulation in wheat (Triticum aestivum L.) grain. Cereal Research Communications, 4, 431–440.
Beevers, L., & Hagman, R. H. (1969). Nitrate reduction in higher plants. Annual Review of Plant Physiology, 20, 495–522.
Bhatt, I. D., Rawal, R. S., & Dhar, U. (2000). The availability, fruit yield and harvest of Myrica esculenta Buch-Ham ex D. Don in Kumaon (West Himalaya). India. Mountain Research and Development, 20(2), 146–153.
Bray, C. M. (1983). Nitrogen metabolism in plants. Harlow: Longman group Ltd, Longman house, Burnt Mill.
Chaukiyal, S. P. (2011). Shifting in flowering behavior of Myrica esculenta Buch-Ham ex D.Don-a report. Indian Forester, 137, 1350–1352.
Chaukiyal, S. P., & Pokhriyal, T. C. (1996). Nitrate assimilation activity and growth pattern in Pongamia pinnata pierre leaf. Annals of Forestry, 4(1), 94–100.
Evans, H. J., & Nason, A. (1953). Pyridine nucleotide nitrate reductase from extracts of higher plants. Plant Physiology, 28, 233–254.
Hageman, R. H., Leng, E. R., & Dudley, (1967). A biochemical approach to plant breeding. Advances in Agronomy, 19, 45–86.
Kanjilal, U. N. (1969). Forest Flora of the Chakrata, Dehradun and Saharanpur Forest Divisions Uttar Pradesh. Gupta, B.L. 3, pp 493.
Klepper, L. D., Flesher, D., & Hageman, R. H. (1971). Generation of reduced nicotinamide adenine dinucleotide for nitrate reduction in green leaves. Plant Physiology, 48, 580–590.
Nair, T. V. R., & Abrol, Y. P. (1977). Studies of nitrate reducing systems in developing wheat ears. Crop Science, 17, 428–442.
Passonneau, J. V., & Lowry, O. H. (1993). Enzymatic analysis: a practical guide (pp. 85–110). Totowa: Humana Press.
Patel, R. K., & De, L. C. (2006). Soh-phie (Myrica species)–an unexploited fruit of the future for Meghalaya. ENVIS Bulletin, 14(1), 3.
Pokhriyal, T. C., & Abrol, Y. P. (1980). Nitrate assimilation in relation to total reduced N in Bengal grasses (Cicer arietinum). Experimental Agriculture, 16, 121–135.
Pokhriyal, T. C., & Raturi, A. S. (1984). Nitrate assimilation in leaf blades of Eucalyptus. Indian Forester, 110(2), 202–208.
Pokhriyal, T. C., & Raturi, A. S. (1985). A study of nitrate reductase activity in the Populus deltoides leaves. Indian Forester, 111(2), 82–89.
Ratrey, A., Chaukiyal, S. P., Bhatia, P., Neelam, & Deol, N. K. (2013). Assessment of nitrate reductase activity in the leaves of Erythrina blakei R. Parker. Indian Journal of Forest, 36(2), 191–196.
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Chaukiyal, S.P., Khatri, N., Bhatia, P. et al. Standardization of in vivo nitrate reductase activity and its pattern in the individual leaf blades of Myrica esculenta Buch. Ham. ex. D. Don. Ind J Plant Physiol. 19, 287–291 (2014). https://doi.org/10.1007/s40502-014-0096-5
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DOI: https://doi.org/10.1007/s40502-014-0096-5