Abstract
Rice is one of the most important cereal crops worldwide. Grain chalkiness has been reported to decrease rice quality and to be associated with starch biosynthesis and accumulation. In order to explore mechanism of chalky starch formation, microscopic and submicroscopic techniques were adopted in this study to observe the amyloplast morphology of rice endosperm cells. These techniques used serial semi-thin sections of rice caryopsis endosperm cells and chalky rice kernels at different development stages after anthesis. Amyloplasts with single-starch granule existed in both developing and mature endosperm cells from their appearance to degradation. In addition, particle size analysis of the chalky and normal starch granules showed that the circularity of the chalky single-starch granule was greater than that of the normal starch granule. Given the loose arrangement of single-starch granule caused by undesirable development, its granule is different from that of the normal endosperm cell. Therefore, the defective development of starch might be related to the chalky formation.
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Chen Y-F, Gu Y-J, Wang Z (2009) Difference in caryopsis development among four rice varieties differing in grain weight. Chin J Rice Sci 23:405–413
Cheng F, Hu D, Ding Y (2000) Dynamic change of chalkiness and observation of grain endosperm structure with scanning electron microscope under controlled temperature condition. Chin J Rice Sci 14:83–87
Dang JMC, Copeland L (2004) Studies of the fracture surface of rice grains using environmental scanning electron microscopy. J Sci Food Agric 84:707–713. doi:10.1002/jsfa.1671
Fang C, Hu X, Sun C, Duan B, Xie L, Zhou P (2015) Simultaneous determination of multi rice quality parameters using image analysis method. Food Anal Methods 8:70–78. doi:10.1007/s12161-014-9870-2
Grass Phylogeny Working G, II (2012) New grass phylogeny resolves deep evolutionary relationships and discovers C4 origins. New Phytol 193:304–312. doi:10.1111/j.1469-8137.2011.03972.x
Ji Q-E, Zheng H-Q, Xu Z-X, Lan S-Y (1998) Observation on the microstructure of endosperm starch granule of rice with different qualities. J Fujian Agric Univ 27:241–244
Kang HG, Park S, Matsuoka M, An G (2005) White-core endosperm floury endosperm-4 in rice is generated by knockout mutations in the C-type pyruvate orthophosphate dikinase gene (OsPPDKB). Plant J 42:901–911. doi:10.1111/j.1365-313X.2005.02423.x
Kim SS, Lee SE, Kim OW, Kim DC (2000) Physicochemical characteristics of chalky kernels and their effects on sensory quality of cooked rice. Cereal Chem 77:376–379. doi:10.1094/CCHEM.2000.77.3.376
Matsushima R (2015) Morphological variations of starch grains. In: Nakamura Y (ed) Starch. Springer, Tokyo, pp 425–441. doi:10.1007/978-4-431-55495-0_13
Matsushima R, Maekawa M, Fujita N, Sakamoto W (2010) A rapid, direct observation method to isolate mutants with defects in starch grain morphology in rice. Plant Cell Physiol 51:728–741. doi:10.1093/pcp/pcq040
Matsushima R, Maekawa M, Sakamoto W (2015) Geometrical formation of compound starch grains in rice implements Voronoi diagram. Plant Cell Physiol 56:2150–2157. doi:10.1093/pcp/pcv123
Myers AM, Morell MK, James MG, Ball SG (2000) Recent progress toward understanding biosynthesis of the amylopectin crystal. Plant Physiol 122:989–997
Odeku OA, Itiola OA (2010) Compaction properties of three types of starch. Ir J Pharm Res 6:17–23
Patindol JA, Siebenmorgen TJ, Wang Y-J (2015) Impact of environmental factors on rice starch structure: a review. Starch Stärke 67:42–54. doi:10.1002/star.201400174
Raju GN, Srinivas T (1991) Effect of physical, physiological, and chemical factors on the expression of chalkiness in rice. Cereal Chem 68:210–211
Shen B (2000) Observation on the starch grain development in endosperm of early indica rice during chalkiness formation with scanning electronic microscope. Chin J Rice Sci 14:225–228
Singh Sodhi N, Singh N (2003) Morphological, thermal and rheological properties of starches separated from rice cultivars grown in India. Food Chem 80:99–108. doi:10.1016/S0308-8146(02)00246-7
Tang S, Jiang Y, Li S, Yu H, Zhang Y (1999) Observation on the amyloplasts in endosperm of early indica rice with scanning electron microscope. Acta Agron Sin 25:269–271
Wei C-X, Zhang J, Zhou W-D, Chen Y-F, Liu Q-Q (2008) Degradation of amyloplast envelope and discussion on the concept of compound starch granule in rice endosperm. Chin J Rice Sci 22:377–384
Yamakawa H, Hirose T, Kuroda M, Yamaguchi T (2007) Comprehensive expression profiling of rice grain filling-related genes under high temperature using DNA microarray. Plant Physiol 144:258–277. doi:10.1104/pp.107.098665
Zhang Q (2007) Strategies for developing green super rice. Proc Natl Acad Sci USA 104:16402–16409. doi:10.1073/pnas.0708013104
Zhou LJ, Jiang L, Zhai HQ, Wan JM (2009) Current status and strategies for improvement of rice grain chalkiness. Yi Chuan 31:563–572
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Liu, Z., Wang, Z. & Zhu, J. Observational studies on amyloplasts with single-starch granule in rice endosperm. Braz. J. Bot 39, 821–832 (2016). https://doi.org/10.1007/s40415-016-0291-x
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DOI: https://doi.org/10.1007/s40415-016-0291-x